Omni Bead Ruptor bead mill homogenizer protocols

Omni Bead Ruptor Bead Mill Homogenizer Protocols for Tissue Homogenization and Cell Lysis

Various tissues and sample types were homogenized and assessed for analytes using the portfolio of Omni Bead Ruptor bead mill homogenizers. Below is our list of resulting performance-verified protocol snapshots, for tissue and cell homogenization protocols and specific bead mill homogenizer protocols. Note that all listed protocols are only recommended starting points from our or external lab experiences, and labs will need to verify their own protocols for their own workflows.

When comparing several commercially available bead mill homogenizers based on maceration level, nucleic acid quality, quantity, amplification, and DNA shearing to determine which instruments were effective for their sample type, the authors’ wrote:

“Upon receiving the highest score on the evaluation matrix, the Bead Ruptor 24 Elite was further tested to determine whether it could consistently disrupt a tick body enough to release detectable quantity of pathogen…. In the present study, the tested traditional bead homogenizer, the Bead Ruptor 24 [Elite], seemed to perform the best overall.”1

Several tissue disruption methods exist to support labs access the molecular analyte they need for their downstream assays and analyses. Some rely on chemical lysis of tough materials and membrane disruption while others rely on mechanical disruption using shearing or impact force. Homogenizers fall into the latter category – leveraging rotor-stator or bead-milling forces to uniformly homogenize starting material. As mechanical disruption does not require the harsh use of chemicals, some researchers prefer to rely on homogenization for either all or part of their tissue disruption methods. These researchers require thorough cell lysis, but wish to keep cellular stress to a minimum, as well as avoid excessive handling of toxic chemicals for their lab personnel.
 

Contact us Technical & Application support Request training


For research use only. Not for use in diagnostic procedures.

Sample Types for Tissue Homogenization and Analysis

Human (Homo sapien) tissue

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Blood 0.8 mL Protein, DNA 0.1 mm ceramic 2 mL tube, screw cap

1. 30 Hz for 2.5 mins, 2 cycles

Note: Flip tube holder 180 ° after each cycle

 Bead Ruptor 96 19-632
Cell Culture (MCF-7, breast cancer cell line) Cell pellet from one 6-well cell culture plate (~500K cells/well) Protein 2.8 mm ceramic 7 mL tube

1. Add 1mL cell lysis buffer to cell pellet and transfer to 2mL tube
2. 4.5 m/s for 20 sec

Download the application note

 Bead Ruptor Elite 19-628
Cell Culture (NP cells, neural progenitor cells) 500 µL of a < 1 x 107 cells / mL suspension Steroids 2.4 mm metal 2 mL reinforced tube

1. 4.2 m/s for 30 sec

Download the application note

 Bead Ruptor Elite 19-627
Contrived fecal samples 25 mg fresh fecal sample spiked with 200 µL viral stock DNA, RNA 25 mm milling ball x 1 50 mL milling jar

1. Add lysis buffer.
2. 4.2 m/s for 30 sec.
3. Centrifuge at 10,000 rpm for 2 min

Download the application note

 Bead Ruptor Elite 19-647
Hair (wet grind) Up to 20 mg Protein 2.8 mm ceramic 2 mL tube

1. Add 1 mL methanol
2. 5.3 m/s for 180 sec, 3 cycles with 20 sec dwell

Download the application note

 Bead Ruptor Elite 19-628, 19-678
Hair (dry grind) 10 – 50 mg Hormones 2.8 mm ceramic 2mL reinforced tube

1. 5.3 m/s for 60 sec, 3 cycles with 20 sec dwell
2. Take an aliquot, add 1mL diluent.
3. 5.3 m/s for 60 sec, 3 cycles with 20 sec dwell

Download the application note

 Bead Ruptor Elite 19-628, 19-678
Fingernails 12 – 15 mg Small molecules 2.8 mm ceramic 7 mL tube

1. Add 400 µL total buffer
2. 6.8 m/sfor 30 sec, 4 cycles with 1 min dwell
3. Centrifuge 10Kxg for 1 min, incubate 37 °C for 1 hr
4. Repeat step 2.
5. Centrifuge 10Kxg 1 min, incubate 70 °C for 10 min, pulse spin to pull down supernatant, proceed with extraction

Download the application note

 Bead Ruptor Elite 19-628
Nitrocellulose wastewater filters Intact filters Small molecules 2.8 mm ceramic 7 mL tube

1. Add 600 µL lysis buffer
2. 6.0 m/s for 60 sec

Download the application note

 Bead Ruptor Elite 19-628
PFA-fixed placenta 200 mg ± 5mg tissue Small molecules 2.4 mm metal 7 mL tube

1. Add 600 µL PBS, pH 7.2
2. 5.0 m/s for 30 secs, 3 cycles with 10 sec dwell
3. Add 600 µL lysis buffer, vortex 10 sec, and proceed to de-crosslink protocol

Download the application note

 Bead Ruptor Elite 19-628
PFA-fixed placenta 200 mg ± 10 mg tissue Bacteria 2.8 mm ceramic 2 mL reinforced tube

1. Add 600 µL PBS
2. 5.0 m/s for 30 secs, 2 cycles with 10 sec dwell

Download the application note

 Bead Ruptor Elite 19-628
Saliva 250 µL saliva with 250 µL of viral transport media RNA 2.8 mm ceramic 2 mL reinforced tube

1. 4.5 m/s for 30 sec

Download the application note

 Bead Ruptor Elite 19-628
Contrived swab samples Saturated sterile cotton swabs (stem removed) in 1 mL of viral transport media mRNA 6.5 mm ceramic 2 mL reinforced tube

1. 4.2 m/s for 30 sec
2. Allow froth in tube to settle prior to unscrewing top

Download the application note for IAV M gene

Download the application note for HCoV-229E N gene

 Bead Ruptor Elite 19-660-1000
Teeth One tooth DNA 2 x 10 mm stainless steel milling ball 25 mL milling jar

1. 20 Hz, 1 min

Note: Place jar in freezer for 10 min prior to use

 Bead Ruptor 96 27-203; 27-004
Post-mortem, preserved tissues, diced and fixed kidney, PBMCs, liver, and brain Small diced pieces Sample prep into fragmentation via sonication to ChIP-seq 5 mm stainless steel (3) 2 mL reinforced tubes

1. 4 m/s for 30 sec

Download the application note

 Bead Ruptor Elite 27-7005, 19-648
Post-mortem, preserved tissues, diced and fixed lung, heart, ovary, uterus, and adipose Small diced pieces Sample prep into fragmentation via sonication to ChIP-seq 5 mm stainless steel (3) 2 mL reinforced tubes

1. 4 m/s for 20 sec, 2 cycles with 2 min dwell

Download the application note

 Bead Ruptor Elite 27-7005, 19-648
PFA-fixed umbilical cord 200 mg ± 5 mg tissue DNA 2.8 mm ceramic 2 mL reinforced tubes

1. Add 600 µL PBS, pH 7.2
2. 5.0 m/s for 30 secs, 3 cycles with 10 sec dwell
3. Add 600 µL lysis buffer, vortex 10 sec, and proceed to de-crosslink protocol

Download the application note

 Bead Ruptor Elite 19-628
PFA-fixed umbilical cord 200 mg mg ± 10 mg tissue Protein 2.8 mm ceramic 2 mL reinforced tubes

1. Add 600 µL PBS, pH 7.2
2. 5.0 m/s for 30 secs, 2 cycles with 10 sec dwell

Download the application note

 Bead Ruptor Elite 19-628

Animal tissue

Mammals
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Bat skin (Artibeus phaeotis) 25 – 100 mg Amino acids 2.8 mm ceramic 7 mL tube 1. 6.8 m/s for 30 sec 
2. Add 3 mL of TCA to tube 
3. 6.8 m/s, 30 secs, 3 additional cycles 

Note: Trichloroacetic acid (TCA) is a protein-precipitating agent.		 Bead Ruptor Elite 19-678
Bull hair (Bos Taurus) 50 mg Steroids 2.4 mm metal 2 mL reinforced tube 1. 6.95 m/s for 50 secs, 2 cycles with 15 sec dwell Bead Ruptor Elite 19-620
Bull bone (Bos Taurus) 5 g DNA, RNA 25 mm milling ball x 1 50 mL milling jar 1. break bone (5g) into shards to fit milling jar with ball. 
2. Place jar in liquid nitrogen (3 mins) 
4. Load and process 25 Hz, 2 mins		 Bead Ruptor 96 27-006, 27-206
Cow/Bovine Articular cartilage 5 mg Protein 2.8 mm ceramic 2 mL tube 1. Add 0.5 mL Tris. 
2. 6 m/s for 30 secs, 2 cycles with 30 sec dwell		 Bead Ruptor Elite 19-628
Coyote hair (Canis latrans) 2-3 mm Hormones 2.8 mm ceramic 2mL reinforced tube 1. 6.8 m/s for 50 secs, 4 cycles Bead Ruptor Elite 19-628
Dog placenta (Canis familiaris) 1 g Small molecules 2.8 mm ceramic 7 mL tube 1. Add 2 mL of water 
2. 6 m/s for 30 sec		 Bead Ruptor Elite 19-678
Horse hair (Equus ferus caballus) 100 mg Small molecules 2.8 mm ceramic 7 mL tube 1. HIGH speed for 30 secs, 5 cycles with 30 sec dwells Bead Ruptor 12 19-678
Horse hair (Equus ferus caballus) 100 mg Small molecules 2.4 mm metal 7 mL tube 1. 6.8 m/s for 50 secs, 3 cycles with 3 min dwells Bead Ruptor Elite 19-670
Mouse lung (Mus musculus ) 200 mg Bacteria 2.8 mm ceramic 2 mL reinforced tube 1. Add 1 mL Tris. 
5.65 m/s for 30 sec, 3 cycles with 5 sec dwell		 Bead Ruptor Elite 19-628
Mouse lung, kidney, liver, heart (Mus musculus ) 25 mg RNA 2.8 mm ceramic 2 mL reinforced tube 1. Add 0.5 mL RLB buffer. 
2. 2.9 m/s for 20 secs		 Bead Ruptor 12 19-628
Mouse quadricep muscle (Mus musculus ) 50 mg mRNA 6.5 mm ceramic 2 mL reinforced tube 1. Add 0.5 mL PBS. 
2. 4.5 m/s for 30 secs, 3 cycles with 20 secs dwell on ice		 Bead Ruptor Elite 19-648, 19-682
Pig cartilage / porcine meniscus 20 mg protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 0.5 mL Tris 
2. 6 m/s for 30 secs, 4 cycles with 30 secs dwell		 Bead Ruptor Elite 19-628
Pig skin (Sus domesticus) 200 mg RNA 2.8 mm ceramic 7 mL tube 1. Add 2 mL Tris. 
2. 6 m/s for 30 secs, 3 cycles with 3 min dwells on ice		 Bead Ruptor Elite 19-678
Pig skin (Sus scrofa domesticus) 10 mm2 RNA 6.5 mm ceramic (one bead) 7 mL tube 1. Add 2.5 mL ice-cold TRIzol™. 
2. 5.5 m/s for 30 secs, 2 cycles with 1 min dwell on ice		 Bead Ruptor Elite 19-651-500, 19-682
Rat fat/adipose tissue (Rattus norvegicus) 500 mg Small molecule 2.8 mm ceramic 7 mL tube 1. Add 3 mL water. 
2. 6 m/s for 30 secs		 Bead Ruptor Elite 19-678
Rat femur (bone) (Rattus norvegicus) 200 mg Small molecule 2.4 mm metal 7 mL 1. HIGH speed for 1 min, 2 cycles with 1 min dwell on ice Bead Ruptor 12 19-670
Rat brain (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris 
2. Speed 4 for 45 secs		 Bead Ruptor 4  19-628
Rat brain (Rattus norvegicus) 70 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS 
2. 4.85 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat brain (Rattus norvegicus) 30 mg per well Protein, DNA 5 mm stainless steel (1 per well) Deep 96-well plate 1. Add 500 µL buffer 
2. Seal plate with sealing mat 
3. 20 Hz for 30 secs		 Bead Ruptor 96 27-7005, 27-520, 27-530
Rat colon (Rattus norvegicus) 3.6 g Small molecule 2.8 mm ceramic 30 mL tube 1. Add 10 mL water
2. 5 m/s for 30 secs, 3 cycles with 1.5 min dwells		 Bead Ruptor Elite 19-6358
Rat heart (Rattus norvegicus) 50 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 1 mL Tris
2. HIGH for 1 min		 Bead Ruptor 12 19-628
Rat heart (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris
2. Speed 4 for 45 secs, 2 cycles with 30 sec dwell		 Bead Ruptor 4 19-628
Rat heart (Rattus norvegicus) 30 mg Protein & DNA 5 mm stainless steel (1 per well) Deep 96-well plate 1. Add 500 µL preferred buffer
2. 20 Hz for 30 sec, 3 cycles		 Bead Ruptor 96 27-7005, 27-520, 27-530
Rat small intestine (Rattus norvegicus) 30 mg DNA 2.8 mm ceramic 2 mL reinforced tube 1. Add 200 µL lysis buffer
2. 5 m/s for 10 sec		 Bead Ruptor Elite 19-628
Rat small intestine (Rattus norvegicus) 30 mg Protein, DNA 5 mm stainless steel (1 per well) Deep 96-well plate 1. Add 500 µL preferred buffer
2. 20 Hz for 30 sec, 2 cycles		 Bead Ruptor 96 27-7005, 27-520, 27-530
Rat liver (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris
2. Speed 4 for 45 secs		 Bead Ruptor 4 19-628
Rat liver (Rattus norvegicus) 70 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS
2. 5.3 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat liver (Rattus norvegicus) 5 g Small molecule 2.8 mm ceramic 30 mL tube 1. Add 15 mL water
2. 5 m/s for 45 sec, 2 cycles		 Bead Ruptor Elite 19-6358
Rat liver (Rattus norvegicus) 3 g Small molecule 2.8 mm ceramic 15 mL tube 1. Add 4 mL water
2. 5.8 m/s for 45 sec		 Bead Ruptor Elite 19-6158
Rat lung (Rattus norvegicus) 68 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS
2. 5.5 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat muscle (Rattus norvegicus) 55 mg Protein, DNA 2.4 mm metal 2 mL reinforced tube 1. Add 750 µL Tris
2. HIGH for 45 secs		 Bead Ruptor 12 19-620
Rat muscle (Rattus norvegicus) 30 mg Protein 2.8 mm ceramic 2 mL reinforced tube 1. Add 750 µL Tris
2. Speed 4 for 45 secs		 Bead Ruptor 4 19-628
Rat muscle (Rattus norvegicus) 50 mg Lipid 2.8 mm ceramic 2 mL reinforced tube 1. Add 1 mL water
2. 5.65 m/s for 1 min, 2 cycles		 Bead Ruptor Elite 19-628
Rat spleen (Rattus norvegicus) 60 mg Protein 1.4 mm ceramic 2 mL reinforced tube 1. Add 500 µL TBS
2. 4.85 m/s for 20 secs		 Bead Ruptor Elite 19-627
Rat trachea (Rattus norvegicus) 2 – 3 mm2 (3 – 10 mg) RNA 1.4 mm ceramic 0.5 mL tubes 1. Add 250 µL Tris
2. Speed 4 for 60 secs, 2 cycles		 Bead Ruptor 4 19-626

 

Birds
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Chicken liver (Gallus gallus domesticus) 2 g Small molecule 2.8 mm ceramic 15 mL tube 1. Add 3 mL of water 
2. 6.45 m/s for 30 secs, 3 cycles with 1 min dwells		 Bead Ruptor Elite 19-6158
Chicken liver (Gallus gallus domesticus) 5 g Small molecule 2.8 mm ceramic 30 mL tube 1. Add 10 mL of water 
2. 5 m/s for 45 secs, 2 cycles		 Bead Ruptor Elite  19-6358
Chicken liver (Gallus gallus domesticus) 200 mg Small molecule 2.8 mm ceramic 7 mL tube 1. Add 1.8 mL 50:50 Methanol:H2O 
2. 5.3 m/s for 30 secs		 Bead Ruptor Elite 19-678
Chicken egg shells (Gallus gallus domesticus) 6 g Nutritional analysis 10 mm milling ball x 4 50 mL milling jar 1. 30 Hz, 2 mins Bead Ruptor 96 27-203, 27-006
Goose feather (Anser anser domesticus) One feather (~100 mg) Steroids 2.8 mm ceramic 7 mL tube 1. Cut up feather to fit tube. 
2. 6.6 m/s for 30 secs, 5 cycles with 3 min dwells		 Bead Ruptor Elite 19-678
Goose feather (Anser anser domesticus) One feather (~100 mg) Steroids 2.8 mm ceramic 7 mL tube 1. Cut up feather to fit tube. 
2. HIGH setting for 30 secs, 5 cycles with 3 min dwells		 Bead Ruptor 12 19-678

 

Fish
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Trout head bones (Oncorhynchus mykiss) 2 g Parasite 2.8 mm ceramic 50 mL tube 1. Add 20 mL of 0.5% pepsin-HCL. 
2. 4.7 m/s for 30 secs, 3 cycles with 2 min dwells		 Bead Ruptor Elite 19-6508

 

Insects
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Beetle (Tenebrio molitor) 30 mg DNA 2.8 mm ceramic 2 mL reinforced tube 1. 350 µL preferred lysis buffer 
2. Speed 4 for 30 sec		 Bead Ruptor 4  19-628
Fruit fly (Drosophila melanogaster) (Whole) One fly (30 - 50 mg) Protein, DNA 1.4 mm ceramic 2 mL tube 1. Add 1 mL Tris 
2. 6 m/s for 30 secs		 Bead Ruptor Elite 19-627
Honey bee (Apis mellifera) (Whole) 4 bees DNA 2.8 mm ceramic 7 mL tube 1. Add 2 mL water 
2. 4 m/s for 30 secs, 2 cycles with 15 sec dwell		 Bead Ruptor Elite  19-678
Mosquito (Aedes aegypti) (Whole) ~50 mg DNA 2.4 mm stainless steel Deep 96-well plate 1. Add 1 mL CTL buffer (Omega-Biotek). 
2. 24 Hz, 5 mins. 

Note: Rotate plate halfway through cycle.		 Bead Ruptor 96 19-640, 27-520, 27-530
Wolf spider (Allocosa apora) (Whole) One spider (240 mg) Protein 1.4 mm ceramic 7 mL tube 1. Add 2 mL Tris
2. 4 m/s for 30 secs		 Bead Ruptor Elite 19-677

 

Nematodes
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Vinegar eel (Turbatrix aceti) 3 mL culture DNA 0.5 mm glass 2 mL reinforced tube 1. Spin down and resuspend in 200 µL preferred lysis buffer
2. Transfer to 2 mL tube with bead media
3. Speed 5 for 20 - 30 sec		 Bead Ruptor 4 19-622

Fungi

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Yeast (Saccharomyces cerevisiae) 3 mL culture DNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 480 µL of LD buffer and lyticase solution
2. Incubate at 30 °C, add 200 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. 5 m/s for 30 secs, 2 cycles with 45 sec dwell		 Bead Ruptor Elite 19-632D
Yeast (Saccharomyces cerevisiae) 40 mL culture RNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 480 µL of LD buffer and lyticase solution
2. Incubate at 30 °C, add 350 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. 4.2 m/s for 30 secs, 2 cycles with 45 sec dwell		 Bead Ruptor Elite 19-632D

Bacteria

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Escherichia coli 15 mL overnight culture Protein 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 1 mL Tris
2. Transfer to 2 mL tube with bead mix
3. Speed 5 for 1 min		 Bead Ruptor 4 19-632D
Escherichia coli 3 mL overnight culture DNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 200 µL Tris-EDTA and lysozyme buffer
2. Incubate at 37 °C, add 100 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. 4.35 m/s for 45 secs		 Bead Ruptor Elite 19-632
Escherichia coli 1.6 mL overnight culture DNA 0.1 mm ceramic 2 mL reinforced tube 1. Spin down and resuspend in 200 µL Tris-EDTA and lysozyme buffer
2. Incubate at 37 °C, add 100 µL lysis buffer + 10 µL antifoam
3. Transfer to 2 mL tube with bead mix
4. Speed 4 for 45 secs		 Bead Ruptor 4 19-632D
Escherichia coli 6 mL overnight culture DNA, RNA 0.1 mm ceramic Deep 96-well plate 1. Spin down and resuspend in 400 µL Tris-EDTA and lysozyme buffer
2. Incubate at 37 °C
3. Split samples, 200 µL for DNA extraction and 200 µL for RNA extraction into wells of 96-well plate with bead mix
4. Add 100 µL lysis buffer + 10 µL antifoam to each well
5. 30 Hz for 2 min		 Bead Ruptor 96 27-6006

Algae

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Green algae
Aka “green yeast”
(Chlamydomonas reinhardtii)		 30 mL over-night culture RNA 0.5 mm glass 2 mL tube 1.Spin down culture
2.Resuspend in 300 μL RNA lysis buffer
3.Transfer to 2 mL tube with beads, add 10 μL antifoam
4.Speed 4 for 30 secs		 Bead Ruptor 4 19-622D

Pharmaceuticals

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Aspirin tablets (enteric coated) 5 g Mean particle size of 23.8 μm 25 mm stainless steel (1) 50 mL jar 1. 25 Hz for 5 mins Bead Ruptor 96 27-006, 27-206
Aspirin tablets (regular) 5 g Mean particle size of 11.2 μm 25 mm stainless steel (1) 50 mL jar 1. 25 Hz for 5 mins Bead Ruptor 96 27-006, 27-206
Exedrin Tablets 5 g Mean particle size of 18.4 μm 25 mm stainless steel (1) 50 mL jar 1. 25 Hz for 5 mins Bead Ruptor 96 27-006, 27-206

Foods

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Dried algae (Bifurcaria bifurcate) 5 – 20 mg DNA 2.8 mm ceramic 1.5 mL tubes 1. 5.5 m/s for 30 sec Bead Ruptor Elite 19-618D
Dried algae (Graularia spp.) 5 – 20 mg DNA 1.4 mm ceramic 1.5 mL tube 1. 5 m/s for 60 sec Bead Ruptor Elite 19-617D
Dried algae (Mastocarpus spp.) 5 – 20 mg DNA 1.4 mm ceramic 1.5 mL tube 1. 5 m/s for 60 sec Bead Ruptor Elite 19-617D
Ground beef 2.5 g Bacteria 2.8 mm ceramic 30 mL tube 1. 5 m/s for 30 sec Bead Ruptor Elite 19-6358D
Edible - Brownies 7 g Small molecules 2.8 mm ceramic 30 mL tube 1. Add 10 mL 100% isopropyl alcohol
2. 4.5 m/s for 30 sec		 Bead Ruptor Elite 19-6358D
Edibles - Brownies 5 g Small molecules 25 mm stainless steel (1) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 25 Hz for 1 min		 Bead Ruptor 96 27-006, 27-206
Edibles - Cookies 7 g Small molecules 2.8 mm ceramic 30 mL tube 1. Add 10 mL 100% isopropyl alcohol
2. 4.5 m/s for 30 sec		 Bead Ruptor Elite 19-6358D
Edibles - Gummy Bears 10 g (~4 pieces) Small molecules 25 mm stainless steel (1) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 25 Hz for 1.5 min		 Bead Ruptor 96 27-006, 27-206
Edibles - Potato Chips 7 g Small molecules 2.8 mm ceramic 30 mL tube 1. Add 10 mL 100% isopropyl alcohol
2. 4.5 m/s for 30 sec		 Bead Ruptor Elite 19-6358D
Edibles - Truffles 7 g Small molecules 2.8 mm ceramic 30 mL tube 1. Add 10 mL 100% isopropyl alcohol
2. 4.5 m/s for 30 sec		 Bead Ruptor Elite 19-6358D

Plants

Seeds and beans
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Cherry tomato seeds (Solanum lycopersicum var. cerasiform) 20 mg RNA 2.8 mm ceramic 2 mL tube 1. 5.5 m/s for 30 sec Bead Ruptor Elite 19-628D
Chili pepper seeds (Capsicum chinens) 20 mg RNA 2.8 mm ceramic 2 mL tube 1. 5.5 m/s for 30 sec Bead Ruptor Elite 19-628D
Corn kernels (Zea mays) 10 g DNA 25 mm stainless steel (1) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 25 Hz for 1.5 min		 Bead Ruptor 96 27-006, 27-206
Dried kidney beans (Phaseolus vulgaris) 10 g DNA 25 mm stainless steel (1) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 20 Hz for 1 min		 Bead Ruptor 96 27-006, 27-206
Lettuce seed (Lactuca sativa) 11 mg Amino acids 2.8 mm ceramic 2 mL tube 1. Add 0.5 mL water
2. 5 m/s for 30 sec		 Bead Ruptor Elite 19-628D
Okra pods (Abelmoschus esculentus) 180 mg Protein 2.8 mm ceramic 7 mL tube 1. 5 m/s for 30 sec Bead Ruptor Elite 19-678D
Peanut seeds (Arachis hypogaea) 5 g Nutritional analysis 10 mm stainless steel (4) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 30 Hz for 3 min		 Bead Ruptor 96 27-006, 27-203
Peas (Pisum sativum) 2 g Protein 2.8 mm ceramic 50 mL tube 1. 5.0 m/s for 30 sec Bead Ruptor Elite 19-6508
Peas (Pisum sativum) 2 g Protein 2.8 mm ceramic 30 mL tube 1. 5.0 m/s for 30 sec Bead Ruptor Elite 19-6358D
Rice seeds (Oryza sativa) 1 g Nutritional analysis 6.5 mm ceramic (3) 7 mL tube 1. 5 m/s for 1 min, 2 cycles with 30 sec dwell Bead Ruptor Elite 19-651-500, 19-682
Rice seeds (Oryza sativa) 10 g DNA 25 mm stainless steel (1) 50 mL jar 1. 20 Hz for 1 min Bead Ruptor 96 27-006, 27-206
Dried soybeans (Glycine max) 5 g (~ 12 beans) DNA 6.5 mm ceramic (3) 7 mL tube 1. 5.5 m/s for 30 sec, 5 cycles Bead Ruptor Elite 19-651-500, 19-682
Tomato seed (Solanum lycopersicum) 3 g RNA 2.4 mm stainless steel, ¼ inch ceramic (1) 30 mL tube 1. Add 15 mL RNA lysis buffer
2. 5 m/s for 30 secs, 5 cycles with 5 min dwells on ice		 Bead Ruptor Elite 19-6350Z
Wheat berries/kernels (Triticum sp.) 1 g Nutritional analysis 6.5 mm ceramic 7 mL tube 1. 5 m/s for 1 min, 2 cycles with 30 sec dwell Bead Ruptor Elite 19-651-500, 19-682
Wheat berries/kernels (Triticum aestivum) 30 – 50 mg Protein 6.5 mm ceramic (1) 2 mL tube 1. 4.5 m/s for 30 secs Bead Ruptor Elite 19-648, 19-682

 

Flower and fruit
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Hemp flower (Cannabis sativa) 30 mg DNA 2.8 mm ceramic beads 2 mL tube 1. Add 500µL CTB buffer
2. 3.7 m/s for 20 sec		 Bead Ruptor 12 19-628D
Hops flower (Humulus lupulus) 1.5 g Pesticides 6.5 mm ceramic (5) 50 mL tube 1. 4 m/s for 30 secs, 5 cycles with 30 sec dwell Bead Ruptor Elite 19-6650, 19-682
Tangerine (Citrus tangerine) 6 g Nutritional Analysis 10 mm stainless steel (4) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 30 Hz for 3 min		 Bead Ruptor 96 27-006, 27-203

 

Leaves and shoots
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Bamboo leaves (Bambusoideae) 100 mg DNA 2.8 mm ceramic beads 2 mL tube 1. Add 0.8 mL DNA lysis buffer
2. 5 m/s for 30 sec, 3 cycles with 30 sec dwells		 Bead Ruptor Elite 19-628D
Bamboo leaves (Bambusoideae) 100 mg DNA 2.8 mm ceramic beads 2 mL tube 1. Add 0.8 mL DNA lysis buffer
2. Using tube holder attachment, process at 30 Hz for 1 min, 6 cycles with 30 sec dwells		 Bead Ruptor 96 19-628D, 27-106
Frozen basil leaves (Ocimum basilicum) 50 g DNA 2.8 mm ceramic beads 2 mL tube 1. Add 1 mL water
2. Using tube holder attachment, process at 30 Hz for 1 min, 2 cycles with 180° tube-holder flip between cycles		 Bead Ruptor 96 19-628D, 27-106
Clover leaves (Trifolium repens) 200 mg DNA 2.8 mm ceramic 7 mL tube 1. Add 1 mL Tris
2. 5.65 m/s for 30 sec		 Bead Ruptor Elite 19-678D
Dried Palm leaves (Arecaceae) 200 mg DNA 1.4 mm and 2.8 mm ceramic 2 mL tube 1. 4.2 m/s for 30 sec Bead Ruptor Elite 19-629
Parsley leaves (Petroselinum crispum) 4 g Nutritional analysis 10 mm stainless steel (4) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 30 Hz for 3 min		 Bead Ruptor 96 27-006, 27-203
Privet leaves (Ligustrum sp.) 80 mg DNA 2.8 mm ceramic 2 mL tube 1. Add 0.5 mL CTAB buffer
2. 5.5 m/s for 30 sec		 Bead Ruptor Elite 19-628D
Sorghum leaves (Sorghum amplum) 100 mg DNA, RNA 2.8 mm ceramic 2 mL tube 1. Add 0.5 mL Tris
2. 5.5 m/s for 30 secs		 Bead Ruptor Elite 19-628D
Spinach leaves (Spinacia oleracea) 2 g Protein 2.8 mm ceramic 50 mL tube 1. Add 20 mL Tris
2. 5 m/s for 60 secs		 Bead Ruptor Elite 19-6508
Spinach leaves (Spinacia oleracea) 2 g Protein 2.8 mm ceramic 30 mL tube 1. Add 20 mL Tris
2. 5 m/s for 60 secs		 Bead Ruptor Elite 19-6358D
Sugarcane shoots (Saccharum officinarum) 100 mg DNA, RNA 2.8 mm ceramic 2 mL tube 1. Add 500 μL Tris
2. 5.5 m/s for 30 secs		 Bead Ruptor Elite 19-628D
Tabacco leaves (Nicotiana tabacum) 2 g Heavy metals 10 mm stainless steel (4) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 30 Hz for 3 min		 Bead Ruptor 96 27-006, 27-203
Yarrow leaves (Achillea millefolium) 4 g Heavy metals 10 mm stainless steel (4) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 25 Hz for 2 mins		 Bead Ruptor 96 27-006, 27-203

 

Roots, Bark/Chips, and Cane
Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Beech bark (Fagus grandifolia) 5 g Heavy metals 25 mm stainless steel (1) 50 mL jar 1. 30 Hz for 3 mins Bead Ruptor 96 27-006, 27-206
Pine chips (Pinus resinosa) 100 mg Aromatics 6.5 mm ceramic (1) 2 mL tube 1. 4.5 m/s for 3.5 mins Bead Ruptor Elite 19-648, 19-682
White potato (Solanum tuberosum) 5 g Nutritional analysis 10 mm stainless steel (4) 50 mL jar 1. Submerge jar in liquid nitrogen for 3 mins
2. 30 Hz for 3 min		 Bead Ruptor 96 27-006, 27-203

Environmental

Matrix Amount Analyte Bead media Vessel Protocol Omni unit Consumable or accessory Cat #
Forest soil 15 g Heavy metals 10 mm stainless steel (4) 50 mL jar 1. 30 Hz for 3 mins Bead Ruptor 96 27-006, 27-203
Georgia clay soil 200 mg DNA 0.7 mm garnet 2 mL tube 1. Add 750 µL lysis buffer
2. 5 m/s for 45 sec		 Bead Ruptor Elite 19-624
Halite crystal (rock salt) 7 g Heavy metals 10 mm stainless steel (4) 50 mL jar 1. 30 Hz for 3 mins Bead Ruptor 96 27-006, 27-203

 

References

Amanda M Jones, Marshall T Van de Wyngaerde, Erika T Machtinger, Edwin G Rajotte, Thomas C Baker, Choice of Laboratory Tissue Homogenizers Matters When Recovering Nucleic Acid From Medically Important Ticks, Journal of Medical Entomology, Volume 57, Issue 4, July 2020, Pages 1221–1227, https://doi.org/10.1093/jme/tjaa006

ON THIS PAGE