Metagenomics

Improving the efficiency of metagenomic analysis

Revvity’s streamlined, automated workflow from DNA extraction to sequence ready libraries for 16S and shotgun sequencing resulting in robust data for species identification and functional profiling.

16S sequencing specifically targets and amplifies the 16S rRNA gene, a highly conserved region in microbial genomes. This method provides information about the taxonomic composition of the microbial community, allowing researchers to identify and classify different species present. It is particularly useful for studying the diversity and abundance of bacteria and archaea. 16S sequencing is cost-effective, provides phylogenetic insights, and is preferred when the focus is on understanding the overall structure and composition of the microbial community.

On the other hand, shotgun sequencing involves sequencing all the genetic material present in a sample, including the genomes of both microorganisms and the host organism(s). This method provides a more comprehensive view of the microbial community, allowing for the analysis of all genes and potential functional capabilities. Shotgun sequencing is preferred when studying complex microbial ecosystems, as it enables the identification of specific genes, functional pathways, and potential interactions within the community. It is particularly useful when the aim is to gain insights into the functional potential and metabolic activities of the microorganisms.

Metatranscriptomics is a powerful technique that focuses on studying the active gene expression of microbes within natural environments. Unlike metagenomics, which identifies microbial presence based on genomic content, metatranscriptomics delves into the functional aspects. By analyzing the metatranscriptome, researchers gain insights into which genes are actively expressed, allowing them to understand the functional profile of microbial communities. This approach has applications in fields such as human gut microbiome research, environmental studies, and biotechnology. Overall, metatranscriptomics provides a window into the microbial world, revealing not only who’s there but also what they’re actively doing.

Improve the efficiency of your 16S, shotgun, and metatranscriptomics sequencing workflows with Revvity’s complete, single source workflows.