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NEXTFLEX Rapid XP V2 DNA-Seq Kit

Revvity kit box for reagents and consumables
NEXTFLEX Rapid XP V2 DNA-Seq Kit
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables

The NEXTFLEX® Rapid XP V2 DNA kit offers a simplified DNA library preparation workflow for sequencing on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX® normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool.

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Feature Specification
Automation Compatible Yes
Product Group DNA-seq
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ビュー Related reagents

The NEXTFLEX® Rapid XP V2 DNA kit offers a simplified DNA library preparation workflow for sequencing on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX® normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool.

View product information ビュー Related reagents
Product Variants
Unit Size: 8 rxns
Part #:
NOVA-5149-21
Unit Size: 48 rxns
Part #:
NOVA-5149-22
Unit Size: 96 rxns
Part #:
NOVA-5149-23
For research use only. Not for use in diagnostic procedures.
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Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
NEXTFLEX Rapid XP V2 DNA-Seq Kit
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
NEXTFLEX Rapid XP V2 DNA-Seq Kit
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables

Supporting products you might need

Do you need Unique Dual Index Barcodes?
Revvity kit box for reagents and consumables
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NEXTFLEX Unique Dual Index Barcodes (8NT index, 1-8)

Part #: NOVA-514150-EVAL16
Do you need UDI-UMI Barcodes?
Revvity kit box for reagents and consumables
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NEXTFLEX UDI-UMI Barcodes (1-8)

Part #: NOVA-734100

Product information

Overview

  • Fully automated normalization & fragmentation
  • Enzymatic fragmentation Library prep with end-repair and A-tailing in one reaction
  • Highly efficient ligation with low adapter dimer formation
  • Efficient: Automated on the Sciclone®  and Zephyr®  NGS workstations
  • Up to 96 UDI-UMI Barcodes now available to improve the detection of rare variants.

The NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.

Additional product information

Main Advantages and Highlights
Highly Efficient Ligation with Low Adapter Formation

The NEXTFLEX Rapid XP v2 DNA-Seq Kit is designed to provide exceptional ligation efficiency, which is crucial for high-quality sequencing results. The kit includes optimized reagents and protocols that ensure the efficient and accurate ligation of DNA fragments. This high ligation efficiency reduces the likelihood of adapter dimers or erroneous sequences even at low input concentrations, leading to more reliable and reproducible data. By maximizing the ligation step, the kit enhances overall workflow efficiency and contributes to the generation of high-quality sequencing libraries.

nextflex rapid xp v2 dna seq kit fig1

Figure 1. Low dimer formation with NEXTFLEX Rapid XP V2 DNA-Seq kit. Different library replicates were prepared using either 500 pg (A) or 1 ng (B) of high-quality genomic DNA as input. Adapter dimer should appear as a peak at ~150 bp.

Included Normalization Beads provide highly consistent results while saving you several hours of time while preparing for sequencing

Normalization Beads offer significant benefits in DNA library preparation sequencing workflows by ensuring consistent and accurate sample preparation. They streamline the process by equalizing the concentration of DNA across different samples, which reduces variability and enhances the reliability of sequencing results. This leads to more efficient and cost-effective sequencing, as it minimizes the need for additional normalization steps and reduces the risk of errors. Overall, Normalization Beads contribute to higher quality data and more reproducible outcomes. 

Figure-Normalization-Beads

Figure 2. Analysis of GC bias from libraries obtained with NEXTFLEX Rapid XP V2 DNA-Seq kit. Results show minimal variability in normalized coverage across 20-60% GC content encompassing ~95% of the human genome (Right: red bars). These are consistent across 1-750 ng of genomic DNA input. Simplify Library Construction with NEXTFLEX Normalization Beads.

nextflex rapid xp v2 dna seq kit fig3

Figure 3. This image illustrates the steps removed and time savings by using Normalization Beads during library preparation

Flexible multiplexing options: Up to 1,536 UDI or 96 UDI-UMI adapters

Barcodes are not supplied with the NEXTFLEX Rapid XP DNA-seq kit v2 and need to be purchased separately. We offer 384 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs and a set of 1,536 NEXTFLEX UDI adapters for ultra high-throughput applications.

NEXTFLEX UDI-UMI adapters, incorporating molecular barcodes into each adapter, are also available for applications involving the detection of rare variants.

Automation Compatibility

The NEXTFLEX Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone G3 NGSx workstation, Sciclone G3 NGSx iQ™ workstation, and Zephyr NGS workstation.

Workflow
RapidXPv2-Workflow

Specifications

Automation Compatible
Yes
Product Group
DNA-seq
Shipping Conditions
Dual Temperature
Unit Size
8 rxns

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Application Note
Low input PCR-free DNA-seq library prep and normalization

With a turn-around time of only 2.5 hours, the NEXTFLEX® Rapid XP DNA-Seq Kit v2 is a convenient workflow that can be used to...

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Rapid XP V2 DNA-Seq Kit Flyer

The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads*, which provide a consistent mass and...

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