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Cell Counting and Image Cytometry

Cell Viability for Clean and Messy Samples

Section
Cellometer Applications
Celigo Applications
Cell Counting Method Selection
Cell Counting and Image Cytometry FAQs
Cell-based Assays for Bioprocessing
Cell-based Assays for Gene Therapy Development
Cellometer Applications
Modern Virology Assays
Sub Section
Cell Viability for Clean and Messy Samples
Automated Analysis of Primary Cells
Brightfield Cell Analysis
Cell Analysis for Breweries and Biofuels
Cell Concentration and Viability for Blood-based Samples
Cell Counting with a Hemocytometer
Cell Viability for Clean and Messy Samples
Fluorescent Assays
Immuno-oncology Research Using Cellometer Instruments
Miscellaneous Cell Types
Topic
Select an option
Alternative Membrane Exclusion Dyes
Comparing Trypan Blue and AO/PI Staining Methods
Induced Necrosis
Viability Using AO/PI
Viability Using PI
Viability Using Trypan Blue
Explore the topics in this section
Viability Using Trypan Blue
Ideal for clean samples, the Trypan Blue exclusion assay provides viability by determining dead cells from total cells counted.
Viability Using AO/PI
Acridine Orange and Propidium Iodide are nuclear stains that are able to provide viability measurements in messy, primary samples.
Comparing Trypan Blue and AO/PI Staining Methods
A head-to-head comparison of viability determined by Trypan Blue and AO/PI.
Induced Necrosis
Measure induced necrosis by staining your cell sample with different membrane exclusion dyes.
Viability Using PI
Directly measure the number of dead cells and percent viability by staining your samples with propidium iodide.
Alternative Membrane Exclusion Dyes
Using 7AAD, SYTOX Green, Ethidium Bromide, SYTOX Red, DRAQ7 and DAPI for viability measurements.


For research use only. Not for use in diagnostic procedures.