Skip to main content
メニュー
JP

1950年代にイムノアッセイが導入されて以来、イムノアッセイは研究や創薬に不可欠な測定ツールとなってきました。長年にわたり、イムノアッセイ技術とフォーマットは拡大進歩し、今日のイムノアッセイ技術プラットフォームには幅広い選択肢があります。

従来の洗浄ベースのアッセイから、洗浄不要のホモジニアスアッセイキットやツールボックス試薬まで幅広い選択肢をご用意しています。サンプルの適合性から、サンプル量、スループット、検出可能範囲、シグナルウィンドウ、シグナルの安定性、再現性、オートメーション適合性、結果が出るまでのスピードなどにおいて優れた結果を得るためのイムノアッセイテクノロジープラットフォーム、アッセイ用キット、カスタマイズ可能な試薬を豊富に取り揃えています。

イムノアッセイプラットフォームソリューションのメリット

  • 広いダイナミックレンジ
  • 高感度
  • バックグラウンドノイズの低減 
  • 大きなストークシフト
  • シグナル安定性
  • 迅速なデータアウトプット
  • マルチプレックスアッセイ


研究用です。診断にはご使用いただけません。

イムノアッセイ

Alpha

Alphaはビーズベースの洗浄不要な発光技術です。AlphaのDonorビーズとAcceptorビーズがターゲットに結合すると、一重項酸素の拡散により増幅された発光シグナルが生成されます。これらのビーズには、ユニークな生化学反応の検出が可能な様々な生体分子が結合しており、分子間相互作用の研究に洗浄操作なしで活用できます。Alphaテクノロジーは、生化学反応溶液、細胞上清、細胞や生体組織の溶解液、血清、血漿、その他の生物学的サンプルまで幅広い種類のサンプルにも適用できます。

Alphaは以下のようなメリットを提供します:

  • 測定時間の短縮
  • 大きなタンパク質複合体、ヌクレオソーム、ウイルス粒子の定量が可能 
  • マルチプレックス測定

Learn more

technology-alphalisa_assay1056px

DELFIA TRF

DELFIA™ TRFは、20年以上の歴史があり、豊富な実績のある洗浄ベースのイムノアッセイプラットフォームです。DELFIAの測定原理とワークフローは従来のELISAと同様ですが、安定した時間分解蛍光シグナルにより感度やダイナミックレンジが向上できるという利点があります。また、DELFIAは異なるランタノイドキレート標識によりマルチプレックス測定も可能です。DELFIA TRFは、生化学反応溶液、細胞上清、細胞や生体組織の溶解液、血清、血漿、その他の生物学的サンプルまで幅広い種類のサンプルにも適用できます。 

Learn more

delfia-assay-principle-assay-protocol-technology-delfia-assay-steps1056px

ELISA

BioLegendのELISAキットとセットで一つのターゲットに集中しましょう。経済的なキット、抗体、補助試薬で ELISA ワークフローに必要な全ての試薬を見つけることができます。BioLegendは4種類のELISAキットを提供しています。使いやすいプレコートプレート付きのLEGEND MAX™キットや、アッセイ時間を90分に短縮するRAPID MAX™キットもあります(どのように時間が節約されるかはプロトコールのビデオを見てください)。さらに、BioLegend は、独自の ELISA アッセイを開発したい方のために、信頼性の高い抗体ペア、補助試薬、標準品を提供しています。

Learn more

HTRF

HTRF™ (Homogeneous Time Resolved Technology)は、FRETベースの洗浄不要の技術です。

HTRFは、迅速、均質、使いやすく、自動化しやすいため、極限までミニチュア化し、アッセイ開発を容易にすることができます。HTRFは、時間分解FRETのゴールドスタンダードとなっています。

HTRF技術は、競合的および非競合的なフォーマットで使用することができ、細胞アッセイまたは生化学的アッセイとして実施できるため、GPCR、キナーゼ、エピジェネティクス、サイトカインを含むさまざまなバイオマーカーの定量化など、さまざまなアプリケーションに適用できます。

Learn more

technology-htrf-assay1056px

LANCE Ultra TR-FRET

LANCE™ Ultra TR-FRETは、Euキレートdonorおよびacceptorの蛍光色素ラベル分子を使用することで、長寿命蛍光の時間分解能(time resolution: TR)の利点とFRETの原理を組み合わせた、洗浄不要の近接アッセイ技術です。LANCE Ultra TR-FRETはバッファー、細胞上清、細胞溶解液サンプルに対応しています。

Learn more

technology-lance-assay1056px

LEGENDplex

LEGENDplex™ ビーズベースイムノアッセイはサンドイッチ ELISA と同じ原理で動作しますが、フローサイトメーターのみを使用して、1 つのサンプルで最大 14 のターゲットを同時に定量できるという利点があります。データは無料の LEGENDplex Data Analysis Software Suite で解析できます。数十種類の既存パネルから選択するか、目的に合わせたカスタムパネルのご相談も承ります。

Learn more

Alpha、TRF、TR-FRETをターゲット領域別に探索

bg-element-listing-page bg-element-listing-page-mobile
Products & Services
Resource Library
Sort by: Recommended
Recommended
Product Name A-Z
Product Name Z-A
1 - 25 of 3390 Products and Services
New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Ultra™ Human Phospho-SIRPα (pan-Tyr) assay is a sandwich immunoassay for quantitative detection of phospho-SIRPα (pan-Tyr) in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-JAK2 (Tyr1008) assay is a sandwich immunoassay for quantitative detection of phospho-JAK2 (Tyr1008) in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Ultra™ Human Total AXIN-2 assay is a sandwich immunoassay for quantitative detection of total AXIN-2 in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-Rb (Thr821) assay is a sandwich immunoassay for quantitative detection of phospho-Rb (Thr821) in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Biotin-Free Human Total SYK assay is a sandwich immunoassay for quantitative detection of total SYK in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Total ERK1/2 assay is a sandwich immunoassay for quantitative detection of total ERK1/2 in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (Tyr525/526) in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Total STAT1 assay is a sandwich immunoassay for quantitative detection of total STAT1 in cellular lysates using Alpha Technology.

New
Total B-RAF Product image

This HTRF kit allows for the cell-based quantitative detection of total B-RAF.

Part Number: 64BRAFTPEG, 64BRAFTPEH
New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Ultra™ Human Total PAX5 assay is a sandwich immunoassay for quantitative detection of total PAX5 in cellular lysates using Alpha Technology.

New
Nucleolin Phospho-Thr84 Product image

This HTRF kit allows for the cell-based quantitative detection of Nucleolin when phosphorylated at Thr84.

Part Number: 64NCLT84PEG, 64NCLT84PEH
New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (Tyr705) in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Human and Mouse Phospho-STAT5 (Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (Tyr694/699) in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-Rb (Thr826) assay is a sandwich immunoassay for quantitative detection of phospho-Rb (Thr826) in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total Moesin assay is a sandwich immunoassay for quantitative detection of total Moesin in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (Tyr701) in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Biotin-Free Human Total CDK1 assay is a sandwich immunoassay for quantitative detection of total CDK1 in cellular lysates using Alpha Technology.

New
AlphaLISA SureFire Ultra Phospho-Protein image

The AlphaLISA™ SureFire® Ultra™ High Performance Human Phospho-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (Tyr641) in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Ultra™ Human Total PAX2 assay is a sandwich immunoassay for quantitative detection of total PAX2 in cellular lysates using Alpha Technology.

New
AlphaLISA Surefire Ultra Total Protein

The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Total STAT6 assay is a sandwich immunoassay for quantitative detection of total STAT6 in cellular lysates using Alpha Technology.

New
IRAK4 Phospho-Thr345 Product image

This HTRF kit allows for the cell-based quantitative detection of IRAK4 when phosphorylated at Thr345.

Part Number: 64IRK4T45PEG, 64IRK4T45PEH
New
CXCL12 Product image

The HTRF CXCL12 kit allows for the simple and rapid quantification of soluble CXCL12 in cell supernatants, providing a fast and no-wash alternative to traditional wash-based ELISA assays. The kit is suitable for the measurement of CXCL12 from both human and mouse cell lines.

Part Number: 62CXCL12PEG, 62CXCL12PEH
New
WRN Product image

This HTRF kit allows for the cell-based quantitative detection of WRN.

Part Number: 64WRNTPEG, 64WRNTPEH
New
YES Phospho-Tyr426 Product image

This HTRF kit allows for the cell-based quantitative detection of YES when phosphorylated at Tyr426.

Part Number: 64YESY4PEG, 64YESY4PEH
New
Total A-RAF Product image

This HTRF kit allows for the cell-based quantitative detection of total A-RAF.

Part Number: 64ARAFTPEG, 64ARAFTPEH
Sort by: Resource Name A-Z
Resource Name A-Z
Resource Name Z-A
1 - 25 of 290 Resources
Infographic
Infographic
A brief history on protein-protein interactions

Proteins and their interactions have a history of keen research and technological innovations. All scientists deciphering the protein interactions today are the heirs to this history and are now contributing to it, how well do you know it? Learn more in this infographic! For research use only. Not for use in diagnostic procedures.

Application Note
Application Note
A comparative study of two immunoassay platforms to determine lentivirus titer for CAR-T development

The main vectors of gene therapy in research are viruses. The most popular tool for gene delivery is a genetically modified lentivirus. Modified lentivirus (HIV-1) vectors retain their ability to infect undivided cells, thereby increasing their ability to transduce a wide variety of cells, including those that are difficult to transduce. This advantage enables the stable long-term expression of a transgene. In immunotherapy, CAR-T cells are manufactured by transducing the CAR gene with an HIV-1 vector in T cells to express a specific chimeric p24 protein on their surface. This allows them to recognize cancer cells and destroy them. These CAR-T cells must be generated individually to treat each patient. This application note demonstrates a comparative quantification of the p24 titer in a lentiviral GFP control sample using Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms. Check out the different sections of this application note: A lentiviral vector that encodes the CAR construct Determination of the efficiency of transient co-transfection by measuring viral titer Detection of the presence of a p24 HIV-1 specific antibody in the sample Quantification of targets present in the sample

Application Note
Application Note
A fast and simple chemiluminescent assay for monitoring of DNA-protein interactions

Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to study protein-DNA interactions. It is a radioactive assay, suitable only for low-throughput applications due to the need for a gel-based separation step. AlphaLISA ™ is a bead-based chemiluminescent technology, as a faster, simpler, and non-radioactive alternative to EMSA. AlphaLISA ™ is highly sensitive, requiring less nuclear extract compared to EMSA, highly reproducible, and suitable for higher throughput applications. Revvity developed an AlphaLISA immunoassay to monitor the presence of specific DNA-binding proteins in nuclear extracts. In this application note, we use HepG2 nuclear extracts to demonstrate the binding of Sp1 and HNF1 transcription factors to tagged oligonucleotides containing required cognate response elements. Read this note to see the results and access detailed data compared to EMSA.

Whitepaper
Whitepaper
A guide to In Vitro Transcription (IVT) and the importance of dsRNA detection

Application Note
Application Note
A guideline for HTRF cell-based phospho-protein data normalization

Get the best out of your phosphorylation assays Combining phospho and total protein assays enables better analysis. This Application Note provides valuable guidelines for efficiently analyzing and interpreting results from such assay combinations. Check out all the tips and examples in features! Features Introduction to phospho and total protein assay relevance Tips for handling and interpreting data Examples from actual experiments

Case Study
Case Study
A novel non-Invasive in vivo tool for the assessment of NASH

Non-alcoholic fatty liver disease (NAFLD) describes a progressive pathology that affects the liver. Fat accumulation causes fatty liver (NAFL) or steatosis to develop, which leads to lipotoxicity and in turn induces liver inflammation and apoptosis, resulting in non-alcoholic steatohepatitis (NASH). NASH can progress to fibrosis and then cirrhosis, which in some cases will lead to hepatocellular carcinoma (HCC). Read this case study to learn how non-invasive preclinical in vivo imaging was used to longitudinally visualize, quantify, and diagnose NASH with the goal of investigating the efficacy of liver fibrosis-preventing drugs on NAFLD progression.

Technical Note
Technical Note
A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay

G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction. This technical note outlines a method for preparing GPCR membrane model extracts from stable cell lines, specifically for use with the HTRF GTP Gi binding assay. Get this technical note and discover: Key Highlights such as the Importance of GPCRs and the advantages of using HTRF GTP Gi Binding Assay Detailed Method with Cell Culture Preparation, Cell Lysis, Membrane Preparation and Assay Optimization For research use only. Not for use in diagnostic procedures.

Application Note
Application Note
AAV capsid detection and characterization with new AlphaLISA assay kits

Reliable, Quantitative, and No-Wash Assay Platform for AAV Capsid Detection AAVs are widely used as viral vectors in human gene therapy implementing recombinant DNA technology. Measurement of AAV concentration in complex biological matrices is essential for and effective manufacturing of AAV gene therapies. Revvity has developed and manufactured a line of immunoassays that detect and quantify AAV capsid with AlphaLISA™ technology. These assays can detect AAV1, AAV2, AAV3B, AAV5, AAV6, AAV8, and AAV9 serotypes, and measure AAV particles present in cell culture media, lysis buffer, and cell lysate. Get your application note to: Learn more about AAV capsid detection kits Access data on detection limit, assay reproducibility, variability, specificity, and recovery from multiple analyte matrices through explained diagrams See details on experimental conditions

Whitepaper
Whitepaper
Addressing the challenges of bispecific antibody characterization with high-throughput platforms

Bispecific antibodies (bsAbs) have emerged as an attractive class of therapeutic agents with the potential to revolutionize the treatment of various disorders. While the concept of bsAbs has been around for several decades, it has taken time for the technology and understanding of antibody engineering to advance to a point where its practical application has become feasible. The development of bsAbs demands intricate screening processes to identify candidates with desired binding properties, ensuring they induce specific biological activities. Additionally, achieving optimal tissue tropism and potency requires careful consideration of factors such as antibody size, affinity, and the implementation of innovative characterization strategies. In this context, this whitepaper explores novel approaches contributing to bsAb characterization, offering real-world examples that highlight their practical applications.

Infographic
Infographic
Addressing the interactome with protein-protein interaction assays

Protein-protein interactions: cover them all with one approach This brochure illustrates the possibilities and versatility of protein-protein interaction studies. It features six relevant examples of various interaction types taken from literature to show you how studies can be handled with the time-resolved fret-based HTRF approach, including virus blockade, receptor/ligand binding, protease activity, and more. Features: Introduction to the stakes of protein-protein interaction research Illustration of 6 published interaction studies involving biologics or small molecules For research use only. Not for use in diagnostic procedures.

Whitepaper
Whitepaper
Adeno-Associated Virus (AAV) Vectors Manufacturing

What Are the Challenges and Solutions? All across the globe, AAVs are getting the attention of scientists and companies working in gene therapy, as they provide the right combination of characteristics that make them one of the most promising vector today. In 2021 only, the global gene therapy market was 4.1 billion USD, and AAV vector therapies made up more than 43% of that market value! Designing and manufacturing AAV vectors is complex, and to be successful, certain challenges must be addressed. This implies monitoring and optimtizing production for a thorough quality control process, including reliable ongoing characterization of process intermediates and the final product. Key features include: Overview and data on the gene therapy market Description of AAV vectors’ genomes and transfer Challenges associated with AAV vectors’ design and manufacturing Analytical methods for AAV vector quality control with a description of innovative no-wash AlphaLISA™ assays

Flyer
Flyer
Advance your biologics research with Revvity solutions

In the dynamic landscape of pharmaceuticals, biotherapeutics research stands out as the fastest-growing sector. Scientists worldwide are on a quest for ever more efficient tools to develop therapeutic proteins. From lead selection to bioprocessing qualification, rigorous analytical characterization is essential. That’s where Revvity steps in. Our comprehensive line of no-wash tools revolutionizes biologics screening, mechanism-of-action studies, and biomanufacturing. With robustness and convenience at the forefront, we empower researchers to accelerate discoveries and drive innovation. Welcome to a new era of precision and efficiency in biotherapeutics research. For research use only. Not for use in diagnostic procedures.

Application Note
Application Note
Advancing K-Ras Targeted Therapies with Innovative Detection Methods

The RAS family of genes, particularly K-Ras, plays a critical role in cancer biology. Despite its notorious difficulty as a therapeutic target, recent breakthroughs have brought new hope in treating cancers driven by K-Ras mutations. Our latest application note delves into innovative approaches to K-Ras inhibition, including small molecule inhibitors, synthetic lethality strategies, and PROTAC® molecules. We also showcase the high specificity and sensitivity of the no-wash HTRF™ K- Ras immunoassay, a cutting-edge tool that offers a reliable and precise method for evaluating K-Ras protein levels, outperforming traditional techniques. Discover how this assay can accelerate your research in targeting the elusive K-Ras.

Application Note
Application Note
Advantages of using our automatable and no-wash HTRF and AlphaLISA kits for CHO HCP detection

Detecting and quantifying HCPs with automatable homogeneous immunoassays During biotherapeutic manufacturing and production, the host cells - a great majority of them being Chinese Hamster Ovary (CHO) cells – produce protein impurities that are called Host Cell Proteins (HCPs). Even if more than 99% of them are removed from the final product, the residual CHO HCPs can induce immunogenicity in individuals or reduce the potency, stability, or effectiveness of a drug. Therefore, to meet regulatory organizations’ guidelines (such as FDA or EMA) on CHO HCP levels, biopharmaceutical companies spend significant amounts of money on tools and strategies for their detection. Illustrated with robust results, this Application Note explains the many ways in which HTRF™ and AlphaLISA™ kits can improve the workflow for CHO HCP detection: Wide antibody coverage Compatibility with most commonly used buffers No cross-reactivity between CHO HCP detection and drug substance Good dilutional linearity and antigen spike recovery

Brochure
Brochure
Alpha assays and reagents catalog

Alpha technolgy enables the rapid and straightforward mesaure of virtually any target. This includes enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules. Explore the vast selection of homogenous Alpha assays and reagents, including AlphaLISA ™ , AlphaLISA ™ SureFire ® Ultra ™ , AlphaScreen ™ , and Alpha Toolbox. For research use only. Not for use in diagnostic procedures.

Guide
Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

Brochure
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire ®   Ultra ™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology and sandwich immunoassays for detecting phosphorylated proteins in cells. Offering a quantitative alternative to Western Blotting, Alpha SureFire assays are automation-friendly, easily miniaturized, and proficient in detecting both endogenous and recombinant proteins. Explore our comprehensive portfolio of SureFire Assays, designed to help you elevate and expedite your drug discovery journey.

Whitepaper
Whitepaper
Alpha technologies for antibody detection and characterization

Many laboratories developing and producing antibodies still rely on traditional enzyme-linked immunosorbent assay (ELISA) to perform clonal selection and characterization. Whilst well established, ELISAs can lack sensitivity and reproducibility, and are difficult to automate. In this white paper, you will learn how Alpha Technologies can provide a faster and simpler workflow for antibody detection and characterization. It will take you through key steps such as antibody clonal selection, measuring antibody affinity and characterizing antibodies, providing examples and demonstrating the advantages Alpha technology can provide as an alternative to ELISA. Download the white paper to discover how to advance your antibody detection and characterization.

Application Note
Application Note
Alpha technology: a fast and sensitive orthogonal approach to cell-based potency assays

Orthogonal systems to cell-based assays are a key requirement in EMA/FDA guidelines for potency estimations and require cross-validation with complementary approaches to prove and strengthen the reliability of results. In this application note published in collaboration with IBR Inc., you will learn: Why Alpha technology represents an ideal cell-free orthogonal system for potency assays How AlphaLISA assays can be used to determine Bevacizumab/VEGF165 potency An example of how to run an AlphaLISA potency assay and the type of data that can be generated Why it is suitable for assessing lot-to-lot consistency and equivalence of Bevacizumab and biosimilars

Technical Note
Technical Note
AlphaLISA Acetyl‑Histone H3 lysine 27 (H3K27ac) cellular detection kit

Quantifying H3K27ac Levels with AlphaLISA Assay In this technical note, you will discover how AlphaLISA immunodetection assay easily detects alterations in acetylated histone H3 lysine 27 (H3K27ac) levels within cellular extracts.

Technical Note
Technical Note
AlphaLISA Acetyl‑Histone H3 lysine 9 (H3K9ac) cellular assay

Quantifying H3K9ac in Cellular Extracts with AlphaLISA This technical note gives you details about how AlphaLISA immunodetection assay monitors changes in the levels of acetylated histone H3 lysine 9 (H3K9ac) in cellular extracts.

Guide
Guide
AlphaLISA assay development guide

The AlphaLISA technology allows the detection of molecules of interest in serum, plasma, cell culture supernatants or cell lysates in a very sensitive, quantitative, reproducible, and user-friendly assay. Almost, any sandwich assay can be developed to detect an analyte of interest. Discover how to simply your immunoassay workflow with this guide! For research use only. Not for use in diagnostic procedures.

Technical Note
Technical Note
AlphaLISA assays for immune checkpoint detection and quantitation

Quantifying Immune Checkpoints: AlphaLISA Biomarker Detection Kits This technical note focuses on the role of checkpoint molecules in cancer immunotherapies. Checkpoint molecules are molecular markers that can regulate an immune system attack on cancer cells. They have gained prominence as promising candidates for immunotherapies. AlphaLISA detection kits are designed to detect and quantify the levels of these molecules in cell culture media, serum, and cell lysates.

Technical Note
Technical Note
AlphaLISA Di-Methyl-Histone H3 lysine 4 (H3K4me2) cellular assay

Decoding Histone Modifications: A Targeted Assay for H3K4me2 In this study, you will find details about an optimized assay designed to quantify changes in H3K4me2 levels following treatment with sodium butyrate and Trichostatin A (TSA), both of which are non-selective histone deacetylase (HDAC) inhibitors. Using a standardized histone extraction procedure, we detect the target mark by employing a biotinylated anti-Histone H3 (C-terminus) antibody and AlphaLISA Acceptor beads conjugated to an antibody specific to the mark.

Technical Note
Technical Note
AlphaLISA Di/Mono-Methyl-Histone H3 Lysine 27 (H3K27me2-1) cellular detection kit

Quantification of H3K27me3 Levels Using AlphaLISA Technology In this technical note discover how AlphaLISA ™ immunodetection assay tracks variations in cellular extract levels of di-methylated histone H3 lysine 27 (H3K27me2-1).