Most recently released targets
In the race to the next big breakthrough you need to have the best tools available. We are constantly working to keep pace with the speed of your innovation to provide you with the products you need.
In the race to the next big breakthrough you need to have the best tools available. We are constantly working to keep pace with the speed of your innovation to provide you with the products you need.
New assays are available in AlphaLISA™ SureFire® Ultra™, AlphaLISA™, and HTRF™ technologies within several different therapeutic areas and research fields.
- Inflammation: CCL17, CXCL12, IL6, IRAK4 Phospho-T345, Nucleolin Phospho-T84 and Total, TNFα
- Neurodegenerative Disease: AXIN-1 Total, LRRK2 Phospho-S1292 and Total
- Oncology + Autoimmunity: A-RAF Total, B-RAF Total, AXIN-1 Total, AXIN-2 Total, c-Kit Total, ERM Phospho, Ezrin/Moesin Total, IRF5 Aggregate, JAK1 Phospho-Y1034/1035, JAK2 Phospho-Y1008, MERTK Phospho-Y749 and Total, Moesin Phospho-T558 and Total, NPM Phospho-T199 and Total, PAX2 Total, PAX5 Total, PAX8 Total, NPM Phospho-T199 and Total, SIRPα Phospho-pan Y and Total, STAT1 Phospho-Y701, STAT6 Phospho-Y461, YES: Phospho-Y426 and Total, WRN Total
Also, we are pleased to introduce a brand-new line of kits: AlphaLISA SureFire® Biotin Free Detection Kits.
- Oncology: Rb Phospho-S780 and Phospho-S807/811 and Phospho-T821 and Phospho-T826 and Total, STAT6 Phospho-Y641 and Total
- Oncology + Autoimmunity: STAT5 Phospho-Y694/699 and Total, SYK Phospho-Y525/526 and Total
- Oncology + Autoimmunity + Neuroscience: STAT1 Phospho-Y701 and Total, STAT3 Phospho-Y705 and Total
- Oncology + Metabolism: AKT1/2/3 Phospho-S473 and Total
- Oncology + Neuroscience: CDK1 Phospho-T14 and Total, ERK1/2 Phospho-T202/Y204 and Total
Newest target videos
Discover brief animated videos providing information on specific targets and research areas that are addressed by our newly released assay kits!
Discover brief animated videos providing information on specific targets and research areas that are addressed by our newly released assay kits!
LRRK2 in Parkinson's disease
Key protein targets in TPD
Targeting tau proteins
Aurora kinases in the cell cycle
The power of cytokines
Autophagy and its potential in therapeutics
HTT in Huntington’s disease pathology
AAVs as gene therapy vectors
Exploring Janus kinases
BRD proteins as therapeutic targets
LC3B: Key marker of autophagy
Targeting the ECM
Explore Alpha, HTRF, LANCE Ultra, and DELFIA assay technologies
Our Alpha, HTRF™, LANCE™ Ultra™ TR-FRET, and DELFIA™ TRF technologies offer alternatives to standard ELISA assay kits, each with a larger dynamic range and higher signal-to-noise ratio. Alpha, HTRF and LANCE Ultra TR-FRET have the added advantage of having no wash or separation steps, saving you valuable time. Discover sandwich assays, protein detection assays, cell based assays, and more!
Our Alpha, HTRF™, LANCE™ Ultra™ TR-FRET, and DELFIA™ TRF technologies offer alternatives to standard ELISA assay kits, each with a larger dynamic range and higher signal-to-noise ratio. Alpha, HTRF and LANCE Ultra TR-FRET have the added advantage of having no wash or separation steps, saving you valuable time. Discover sandwich assays, protein detection assays, cell based assays, and more!
Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to the target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable the detection of unique biological events, offering maximum flexibility.
HTRF (Homogeneous Time Resolved Technology) is a FRET-based, no-wash technology. HTRF technology is rapid, homogeneous, easy to use and to automate, allows extreme miniaturization and easy assay development. HTRF technology can be used in competitive and noncompetitive formats, performed as cellular or biochemical assays, and thus can been applied to a variety of applications.
LANCE (Lanthanide Chelate Excite) Ultra TR-FRET is a no wash proximity assay that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels.
DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improving assay dynamic range. DELFIA also offers a multiplexing capability.
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This HTRF kit allows for the cell-based quantitative detection of Nucleolin when phosphorylated at Thr84.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-Rb (Thr821) assay is a sandwich immunoassay for quantitative detection of phospho-Rb (Thr821) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Total ERK1/2 assay is a sandwich immunoassay for quantitative detection of total ERK1/2 in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Ultra™ Human Total PAX5 assay is a sandwich immunoassay for quantitative detection of total PAX5 in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Ultra™ Mouse Phospho-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (Tyr641) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Total STAT1 assay is a sandwich immunoassay for quantitative detection of total STAT1 in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (Tyr525/526) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human Total SYK assay is a sandwich immunoassay for quantitative detection of total SYK in cellular lysates using Alpha Technology.
This HTRF kit allows for the cell-based quantitative detection of total B-RAF.
The AlphaLISA™ SureFire® Ultra™ Human Phospho-SIRPα (pan-Tyr) assay is a sandwich immunoassay for quantitative detection of phospho-SIRPα (pan-Tyr) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Ultra™ Human Total AXIN-2 assay is a sandwich immunoassay for quantitative detection of total AXIN-2 in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-STAT3 (Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (Tyr705) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-JAK2 (Tyr1008) assay is a sandwich immunoassay for quantitative detection of phospho-JAK2 (Tyr1008) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human Phospho-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (Ser473) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human Total CDK1 assay is a sandwich immunoassay for quantitative detection of total CDK1 in cellular lysates using Alpha Technology.
This HTRF kit allows for the cell-based quantitative detection of IRAK4 when phosphorylated at Thr345.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (Tyr701) in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Ultra™ High Performance Human Phospho-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (Tyr641) in cellular lysates using Alpha Technology.
The HTRF CXCL12 kit allows for the simple and rapid quantification of soluble CXCL12 in cell supernatants, providing a fast and no-wash alternative to traditional wash-based ELISA assays. The kit is suitable for the measurement of CXCL12 from both human and mouse cell lines.
The AlphaLISA™ SureFire® Biotin-Free Human Phospho-CDK1 (Thr14) assay is a sandwich immunoassay for quantitative detection of phospho-CDK1 (Thr14) in cellular lysates using Alpha Technology.
This HTRF kit allows for the cell-based quantitative detection of WRN.
The AlphaLISA™ SureFire® Ultra™ Human Total PAX2 assay is a sandwich immunoassay for quantitative detection of total PAX2 in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human Total Rb assay is a sandwich immunoassay for quantitative detection of total Rb in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total AXIN-1 assay is a sandwich immunoassay for quantitative detection of total AXIN-1 in cellular lysates using Alpha Technology.
The AlphaLISA™ SureFire® Biotin-Free Human and Mouse Phospho-Rb (Thr826) assay is a sandwich immunoassay for quantitative detection of phospho-Rb (Thr826) in cellular lysates using Alpha Technology.
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Guidelines for optimizing tyrosine phosphorylation detection: Effective use of pervanadate in kinase small molecule inhibitors studies
Introducing AlphaLISA SureFire® Biotin Free: A Versatile Immunoassay Platform for Biotin-Rich Samples.
Species compatibility for HTRF, AlphaLISA SureFire Ultra and Alpha SureFire Ultra Multiplex assays
