Cytokine Assay Reagents

The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to multiplexed detection of a broad range of proteins, molecules and biomarkers. Using a universal, streptavidin-coated Donor bead, multiple AlphaPlex Acceptor beads targeted to various analytes are combined in a single assay well. Download this guide to learn how to easily set up your AlphaPlex assay and streamline your workflow! For research use only. Not for use in diagnostic procedures.

Leptospirosis is an infectious disease caused by Leptospira bacteria, which can spread across different species of mammals. Transmission occurs through contact or consumption of food from infected domestic animals. In cattle, Leptospira infection leads to reproductive failure, affecting productivity and profitability in the dairy and beef industries. Vaccinations are widely used to prevent infection in animals and reduce transmission to humans. This application note: demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigen stimulated PBMCs isolated from cow blood shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.

Immunoassays are a mainstay for the quantification of a variety of biomolecular analytes in drug discovery, drug development, and life sciences research. AlphaLISA® proves advantageous to ELISAs, offering a novel, homogenous immunoassay technology that eliminates wash steps. Using the JANUS® Automated Workstation in combination with AlphaLISA provides a solution to easily preparing assays that can be tailored to the needs of the laboratory. Moreover, the JANUS workstation can be easily set-up to process different assay types in a multi-user, multi-assay environment, thus providing flexible automated workflows.

AlphaLISA™ technology is a highly sensitive, easy-to-use, and reproducible method for detecting and quantifying molecules in various biological matrices. It works by using streptavidin-coated Donor beads and biotinylated anti-analyte antibodies. When these come into close proximity, the excitation of the Donor beads at 680 nM triggers an energy transfer cascade in the Acceptor beads, generating a sharp emission peak at 615 nM. However, some cell culture media contain high levels of biotin, which can interfere with AlphaLISA and other assay technologies that rely on a streptavidin-biotin binding event for detection. High levels of free biotin in the sample matrix can result in a decrease in total counts, lower signal to background ratios, and reduced AlphaLISA assay detection limits. To mitigate this, AlphaLISA biotin-free kits have been developed. This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.

Get a useful overview of today’s immunity knowledge with this booklet Immunity is a collection of complex processes involving multiple strategies and specialized cell types. This booklet provides you with critical information regarding their roles, characteristic and signaling pathways as well as the collaborative behaviors that contribute to immunity. Featured in this guide: Review the fundamentals of immune cell types and mechanisms Learn from a cutting-edge research report Pathways and functional details on over 10 specialized immune cells

Unlock the potential of whole blood analysis for a more accurate and physiologically relevant assessment of biomarkers. Whole blood encompasses all the natural components of blood, offering an environment that enhances cell viability and simplifies the analytical process by reducing the need for extensive sample preparation. This comprehensive matrix allows for a better understanding of cytokine profiles and other critical biomarkers directly in their native state. For researchers utilizing whole blood samples or seeking to integrate this method into their work, Revvity's AlphaLISA™ technology offers a highly sensitive, homogeneous assay for efficient and precise biomarker detection in whole blood. Download this application note to explore how AlphaLISA can streamline your biomarker analysis, delivering reliable results while overcoming the typical challenges associated with whole blood samples.

Cytokine Detection Using AlphaLISA Biotin-Free assays This technical note describes the use of AlphaLISA biotin-free kits for detecting and quantifying cytokines, including interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6), in supernatants produced by human PBMCs cultured for two days with or without Dynabead ® stimulation.

AlphaLISA and LANCE (TR-FRET) biomarker assays can be used to measure ECM-associated protein modulation caused by human transforming growth factor-beta (TGF-β) induction of EMT in a 3D Spheroid model of human prostate carcinoma.

Our R&D scientists were determined to enable your research by developing new and improved versions of our AlphaLISA cytokine assays, leading to quicker, more sensitive results and faster breakthroughs.

The definitive guide to setting up a successful cytokine assay Many therapeutic areas require an understanding of cytokine release. When preparing for a cytokine assay, many underappreciated parameters (e.g. sample handling, cell culture format, sample dilutions) can in fact greatly impact the performance of the cytokine detection. This guide reviews the latest knowledge surrounding the proper use of HTRF cytokine assays. A review of the key terms and definitions in cytokine detection A list of optimization steps Recommendations for data analysis

Ask real blood for real responses Fresh blood is the model of choice to study drug immunotoxicity and predict adverse effects. Written in collaboration with Blood Assay Solutions, this note provides guidelines for fresh blood cytokine quantification. Discover the power of our cytokine portfolio for your research. Features Step by step protocols for fresh blood cytokine quantification in your research Examples of pathway stimulation assays (TCR, TLR …) Comparison beteen fresh blood and PBMCs

Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run cytokine assays, it is key to consider a multitude of factors that impact the assay performance, such as sample dilutions and cell culture format. Utilize this guide to optimize your AlphaLISA ™ cytokine assays with in-depth data and information!

Discover a rapid and reliable tool for the identification and characterization of STING-targeting compounds. Quantifying human IFN-β using an HTRF-based approach This note demonstrates how using HTRF to quantify human IFN-β is a powerful and effective method for the characterization of STING agonists. It provides data demonstrating that the quantification of IFN-β using HTRF is well correlated with ELISA assays. This note also provides data about gene reporter assay correlation with our HTRF IFN-β assay.

Emerging pathways to neuroinflammation and neurodegeneration Neurodegenerative diseases, such as amyotrophic lateral sclerosis, Parkinson's disease, or Alzheimer's disease, occur as a result of neuroinflammation and neurodegenerative progression. For the moment, these diseases are incurable. However, as research progresses, many similarities between these diseases have been found at a sub-cellular level. Review the fundamentals of the neuroinflammation process Learn from a cutting-edge research report Detailed insight into neurodegenerative diseases

Although every cancer patient has the ability to respond to immunotherapy, tumors often fail to regress due to either primary or acquired resistance. In our interview, Dr. Siwen Hu-Lieskovan, PhD, MD, Director of Solid Tumor Immunotherapy at the Huntsman Cancer Institute, University of Utah discussed the mechanisms behind immune evasion and how this understanding can help create a personalized treatment strategy with the goal of prolonged survival.

Dive deeper into fibrosis research In fibrotic disorders, the normal regulation of the extracellular matrix (ECM) is compromised, which leads to excess of ECM or lack of MMPs. This can further lead to a disorganized accumulation of ECM which can ultimately result in organ rigidity and fibrosis. For this reason, the ECM constitutive proteins, MMPs and fibroblasts activating and regulatory mediators such as TGF-β1 and PGE2 are hot spots of therapeutic research for fibrosis. With this review, you will: Better understand how COX enzymes regulate PGE2 levels in TLR stimulated fibroblasts Learn how an AlphaLISA protein-protein interaction assay can be used as a screening platform for TG2-Fibronectin inhibitor identification Discover how CKD might impact the malignant myocardial fibrosis factor combination

A comprehensive overview of fibrosis development Fibrosis is a main contributor to a wide range of organ failures which stems from a mis-regulation of inflammation in response to injury. Fibrosis occurs when the long-term remodeling and removal of ECM suffers from a higher production than removal rate. This event is associated with chronic inflammation, which keeps myofibroblasts active for extended periods, thus making them and their ECM protein secretion the main effectors of pathological fibrosis. This guide is intended to help scientists and researchers appreciate and navigate the cellular and signaling actors of the development of fibrotic pathologies and disorders. Review the fundamentals of fibrosis as a process. A look at TGF-β1 signaling pathways as therapeutic approaches for fibrotic disorders. Insight into immune cell types involved and treating and reversing progressive.

PBMCs: Waste no more There is no reason to waste precious, hard-to-obtain unused cells! This Technical Note shares our internal protocol to freeze and thaw PBMCs to obtain cells every time you need them. These guidelines are a great companion to our Technical Note, “Guidelines for PBMC isolation.” Don’t throw away your cells! Features: PBMC freeze/thaw protocol Validation data

Many scientists have been trying to find ways to utilize cytokines for therapeutic purposes or to monitor their levels when developing new treatments. One crucial factor in this process is the ability to accurately measure cytokine secretion in PBMC using a reliable immunoassay. The AlphaLISA ™ immunoassays offer a fast, no-wash method for detecting cytokines in both simple and complex sample matrices. Discover experimental procedures and high-quality data representing the newest generation of High Performance Cytokine Detection Kits in this application note.

Over the last decade, therapeutic antibodies have emerged as the predominant class of new drugs. Assays to functionally characterize and evaluate antibodies can be used in the discovery process as well as during the development stages of antibody therapeutics. In this detailed case study, we show a suite of assays that can be used to compare an engineered antibody (adalimumab Fc Silent ™ ) to the original therapeutic antibody (Adalimumab). In this application note, discover how Alpha, HTRF and LabChip ® Protein Express assays can help when you are: Comparing the binding affinities of engineered antibody or original therapeutic antibody to the target or Fc receptors, or for readouts in functional cell-based assays Assessing binding capabilities after thermal stress, to check antibody stability Looking for critical data to build confidence in a biosimilar or an engineered therapeutic antibody

Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as well as the pathology of many diseases including cancer, autoimmune conditions, neurodegenerative disorders, and atherosclerosis. As such, cytokines are often used either directly or indirectly as targets of drug discovery for the development of new immunomodulatory therapies. In this application note, we demonstrate how to easily detect and quantify three cytokines, interferon gamma (IFNγ), interleukin 2 (IL-2), and tumor necrosis factor alpha (TNFα) with AlphaLISA ™ High Performance detection kits. This offers a homogenous, sensitive, and fast assay for furthering your cytokine research. Learn more in this detailed application note!

Fibrotic disorders are complex diseases believed to stem from uncontrolled and excessive wound healing processes. The exact causes of these disorders are difficult to determine due to the intricate regulation of inflammation involved. Understanding these mechanisms is crucial as organ fibrosis, a life-threatening condition, can currently only be treated with transplants. This note gathers publications that exemplify various applications of HTRF ™ and AlphaLISA ™ assays to advance fibrosis related research and shows how other researchers have harnessed these technologies to move their studies forward.

Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a comprehensive range of assays designed to meet the needs of drug discovery and biomolecular research. This catalog includes: Phosphorylation cellular assays GPCRs assays Cytokine assays Immunity and cancer assays Biomarkers for main disease areas Tools for PPIs and assay development Kinase biochemical assays Epigenetics assays Biotherapeutics assays Custom Solutions HTRF™ technology in details For research use only. Not for use in diagnostic procedures.

Based on the TR-FRET immunoassay, HTRF technology simplifies your lab life, offering a multitude of advantages that save you both time and money. Discover how the technology works, the advantages of using HTRF, and the wide range of targets and applications, all presented in this infographic. For research use only. Not for use in diagnostic procedures.

This guide provides you an overview of HTRF applications in several therapeutic areas.