AlphaLISA Human IL-10 High Performance Detection Kit, 500 Assay Points

Human Interleukin 10 (IL-10) is a homodimer composed of two subunits of 18 kDa each. It is produced by various T-cell populations, monocytes, macrophages, and different cell types in the liver when stimulated by endogenous or exogenous factors such as stress, exotoxins, TNF-α, and catecholamines. IL-10 inhibits interferon synthesis in Th1 cell clones, monocytes, and macrophages. IL-10 also inhibits antigen presentation to T-cells and IL-12 production by monocytes. It also impairs the proliferation and cytokine synthesis of CD4+ T cells, without having a direct inhibitory effect on CD8+ T cells. On the other hand, IL-10 has a stimulatory effect on B cells, prevents apoptosis and enhances proliferation and differentiation of plasma cells, and inhibits the release of various chemokines by neutrophils. The main biological function of IL-10 is to limit and terminate the inflammatory responses, block proinflammatory cytokine secretion and regulate the differentiation and proliferation of several immune cells. IL-10 activity is mediated by the heteromeric IL-10 receptor (IL-10R), and signals through the tyrosine kinases JAK1 and TYK2, and STATs.

Formats

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.