NEXTFLEX™ Unique dual index barcodes (10NT, 1-16)

NGS Multiplexing Performance

Scale from 2 to 1,536 libraries in a single NovaSeq^® (Illumina) or AVITI^® (Element)flow cell. The 10NT UDI set supports barcode rotation between runs, eliminating carry-over and virtually stopping index hopping on patterned flow cells. Adapters work out-of-the-box with all TruSeq-style library-prep kits, including NEXTFLEX, Illumina, Watchmaker, Twist library prep kit s, whether PCR-free or PCR-amplified.

“Revvity has a set of 1536 validated UDI adapters that we have been regularly using for 5 years. Using the Illumina® NovaSeq® X Plus with this UDI set from Revvity we can sequencing 12,288 samples across 8 lanes of the 10B or 25B flow cells.  This dramatically lowers our sequencing cost per sample!”

-Dr. Charlie Johnson, Director of Texas A&M Agri Life Genomics and Bioinformatics Service

NGS Adapters with High Data Integrity

Each NEXTFLEX UDI adapter has a dedicated 10NT i7 & i5 sequence separated from every other pair by a ≥ 3-base Hamming distance, enabling single-base error correction during demultiplexing. Because the adapter already contains the full flow-cell and primer-binding sites, PCR enrichment is optional. On patterned flow cells (Illumina NovaSeq^®, HiSeq^® 3000/4000, HiSeq^® X, Element AVITI™) the use of true, unique dual indexes cuts index-hopping artefacts to background levels by removing mis-assigned reads. Every lot is sequenced for ≥ 99.9 % index purity and functional ligation efficiency, ensuring data integrity run after run.

Selected Citations that Reference the Use of the NEXTFLEX UDI Barcodes:

• Adolfi, A., Gantz, V.M., Jasinskiene, N. et al. Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi. Nat Commun 11, 5553 (2020).
• Estermann, M. (2020). Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. doi:10.1242/prelights.18906.
• Gardner, E. J., Prigmore, E., Gallone, G., Danecek, P., Samocha, K. E., Handsaker, J., . . . Hurles, M. E. (2019). Contribution of retrotransposition to developmental disorders. Nature Communications, 10(1). doi:10.1038/s41467-019-12520-y.
• Gaeta, N. C., Bean, E., Miles, A. M., Daniel Ubriaco Oliveira Gonçalves De Carvalho, Alemán, M. A., Carvalho, J. S., . . . Ganda, E. (2020). A Cross-Sectional Study of Dairy Cattle Metagenomes Reveals Increased Antimicrobial Resistance in Animals Farmed in a Heavy Metal Contaminated Environment. Frontiers in Microbiology, 11. doi:10.3389/fmicb.2020.590325.
• Hirose, K., Chang, S., Yu, H., Wang, J., Barca, E., Chen, X., . . . Huang, G. N. (2019). Loss of a novel striated muscle-enriched mitochondrial protein Coq10a enhances postnatal cardiac hypertrophic growth. doi:10.1101/755793.
• Leon, K. E., et al. (2020) DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A. bioRxiv 2020.08.21.258020; doi: 10.1101/2020.08.21.258020
• Miura, H., Takahashi, S., Shibata, T. et al. Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing. Nat Protoc 15, 4058–4100 (2020). doi.org/10.1038/s41596-020-0378-5
• Starr, T. N., Greaney, A. J., Hilton, S. K., Crawford, K. H., Navarro, M. J., Bowen, J. E., Bloom, J. D. (2020). Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding. doi:10.1101/2020.06.17.157982.
• Veenvliet, J. V., et al. (2020) Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. bioRxiv 2020.03.04.974949; doi.org/10.1101/2020.03.04.974949.
• Dubey SK et al. (2025). Deciphering age-related transcriptomic changes in the mouse retinal pigment epithelium. Aging (Albany NY) 17(3):657–684. DOI: 10.18632/aging.206219
• Pujari & Cullen (2024) Modulators of MAPK pathway activity during filamentous growth in Saccharomyces cerevisiae. G3 14(6):jkae072
• Kant et al. (2023) High-quality full genome assembly of historic Xylella fastidiosa strains… Microbiol Resour Announc. 12(11):e00536-23.
• Arras et al. (2023) Characterisation of an E. coli line that completely lacks ribonucleotide reduction… eLife 12:e83845.
• Larkin et al. (2025) Climate-driven succession in marine microbiome biodiversity and biogeochemical function. Nat Commun. 16:3926.
• Klauer et al. (2024) Hydrophobins from Aspergillus mediate fungal interactions with microplastics. bioRxiv preprint 2024.11.05.622132.