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NEXTFLEX™ Unique dual index barcodes (10NT, 1-16)

Revvity kit box for reagents and consumables
NEXTFLEX™ Unique dual index barcodes (10NT, 1-16)
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
The NEXTFLEX® Unique Dual Index Barcodes (1-1,536) are 1,536 UDI barcode adapters with 10 nt indexes. These adapters are designed for multiplexing of NGS libraries on all Illumina® and Element® sequencers and are compatible with any TruSeq® style library prep kit. The adapters are available off-the-shelf in sets of 16, 24, 48, 96, 384, or 1536 in formats of one or two reactions per well.
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Feature Specification
Automation Compatible Yes
Product Group Barcodes
The NEXTFLEX® Unique Dual Index Barcodes (1-1,536) are 1,536 UDI barcode adapters with 10 nt indexes. These adapters are designed for multiplexing of NGS libraries on all Illumina® and Element® sequencers and are compatible with any TruSeq® style library prep kit. The adapters are available off-the-shelf in sets of 16, 24, 48, 96, 384, or 1536 in formats of one or two reactions per well.
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Product Variants
Barcodes: 1 - 16
フォーマット
1 rxn per well
Unit Size
16 rxns
Part #:
NOVA-634100-EVAL16
Barcodes: 1 - 24
フォーマット
1 rxn per well
Unit Size
24 rxns
Part #:
NOVA-634100-EVAL24
Barcodes: 1 - 48
フォーマット
1 rxn per well
Unit Size
48 rxns
Part #:
NOVA-634100-EVAL48
Barcodes: 1 - 96
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634101
Barcodes: 97 - 192
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634102
Barcodes: 193 - 288
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634103
Barcodes: 289 - 384
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634104
Barcodes: 385 - 480
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634105
Barcodes: 481 - 576
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634106
Barcodes: 577 - 672
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634107
Barcodes: 673 - 768
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634108
Barcodes: 769 - 864
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634109
Barcodes: 865 - 960
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634110
Barcodes: 961 - 1056
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634111
Barcodes: 1057 - 1152
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634112
Barcodes: 1153 - 1248
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634113
Barcodes: 1249 - 1344
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634114
Barcodes: 1345 - 1440
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634115
Barcodes: 1441 - 1536
フォーマット
1 rxn per well
Unit Size
96 rxns
Part #:
NOVA-634116
Barcodes: 1 - 1536
フォーマット
1 rxn per well
Unit Size
1,536 rxns
Part #:
NOVA-634100
Barcodes: 1 - 384
フォーマット
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534101
Barcodes: 385 - 768
フォーマット
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534102
Barcodes: 769 - 1152
フォーマット
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534103
Barcodes: 1153 - 1536
フォーマット
2 rxns per well
Unit Size
768 rxns
Part #:
NOVA-534104
Barcodes: 1 - 1,536
フォーマット
2 rxns per well
Unit Size
3,072 rxns
Part #:
NOVA-534100
For research use only. Not for use in diagnostic procedures.
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Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
NEXTFLEX™ Unique dual index barcodes (10NT, 1-16)
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables
NEXTFLEX™ Unique dual index barcodes (10NT, 1-16)
Revvity kit box for reagents and consumables
Revvity kit box for reagents and consumables

Product information

Overview

NEXTFLEX® UDI Barcodes (1-1,536) for Ultra High-Throughput Multiplexing
  • Compatible with PCR-free workflows
  • Designed to minimize index hopping that can be present with certain patterned flow cells.
  • Up to 1,536 (10-nt) adapters available for purchase off-the-shelf
  • Every lot is functionally validated and tested for index purity by sequencing
  • NEXTFLEX® Universal Blockers compatible with the NEXTFLEX® UDI Barcodes (1-1,536) are also available

Additional product information

Highest Sample Multiplexing Options for Illumina Sequencers

The design of the NEXTFLEX Unique Dual Index barcodes allow a broad range of multiplexing – users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX Unique Dual Index barcodes are compatible with NEXTFLEX library prep kits designed for paired-end and single-read sequencing, or other workflows for Illumina sequencers that involve the ligation of adapters to adenylated fragments.

“Revvity has a set of 1536 validated UDI adapters that we have been regularly using for 5 years. Using the Illumina® NovaSeq® X Plus with this UDI set from Revvity we can sequencing 12,288 samples across 8 lanes of the 10B or 25B flow cells.  This dramatically lowers our sequencing cost per sample!”

-Dr. Charlie Johnson, Director of Texas A&M Agri Life Genomics and Bioinformatics Service

Highest Level of Data Integrity

The NEXTFLEX Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina NovaSeq®, HiSeq® 3000/4000, and HiSeq X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is tested for index purity and functionality by sequencing.

nextflex udi barcodes infographic wide

 

Specifications

Automation Compatible
Yes
Barcodes
1 - 16
Format
1 rxn per well
Product Group
Barcodes
Shipping Conditions
Shipped in Dry Ice
Unit Size
16 rxns

References

Selected Citations that Reference the Use of the NEXTFLEX UDI Barcodes:

  • Adolfi, A., Gantz, V.M., Jasinskiene, N. et al. Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi. Nat Commun 11, 5553 (2020). https://doi.org/10.1038/s41467-020-19426-0
  • Estermann, M. (2020). Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. doi:10.1242/prelights.18906.
  • Gardner, E. J., Prigmore, E., Gallone, G., Danecek, P., Samocha, K. E., Handsaker, J., . . . Hurles, M. E. (2019). Contribution of retrotransposition to developmental disorders. Nature Communications, 10(1). doi:10.1038/s41467-019-12520-y.
  • Gaeta, N. C., Bean, E., Miles, A. M., Daniel Ubriaco Oliveira Gonçalves De Carvalho, Alemán, M. A., Carvalho, J. S., . . . Ganda, E. (2020). A Cross-Sectional Study of Dairy Cattle Metagenomes Reveals Increased Antimicrobial Resistance in Animals Farmed in a Heavy Metal Contaminated Environment. Frontiers in Microbiology, 11. doi:10.3389/fmicb.2020.590325.
  • Hirose, K., Chang, S., Yu, H., Wang, J., Barca, E., Chen, X., . . . Huang, G. N. (2019). Loss of a novel striated muscle-enriched mitochondrial protein Coq10a enhances postnatal cardiac hypertrophic growth. doi:10.1101/755793.
  • Leon, K. E., et al. (2020) DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A. bioRxiv 2020.08.21.258020; doi: 10.1101/2020.08.21.258020
  • Miura, H., Takahashi, S., Shibata, T. et al. Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing. Nat Protoc 15, 4058–4100 (2020). doi.org/10.1038/s41596-020-0378-5
  • Starr, T. N., Greaney, A. J., Hilton, S. K., Crawford, K. H., Navarro, M. J., Bowen, J. E., Bloom, J. D. (2020). Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding. doi:10.1101/2020.06.17.157982.
  • Veenvliet, J. V., et al. (2020) Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites. bioRxiv 2020.03.04.974949; doi.org/10.1101/2020.03.04.974949.

FAQs

  • Are the NEXTFLEX unique dual index barcodes sequence-verified for purity?
  • Are the NEXTFLEX unique dual index barcodes functionally validated?
  • What is the lead time when I order the NEXTFLEX unique dual index barcodes?
  • Why are the NEXTFLEX unique dual index barcodes only supplied at two reactions of each barcode in the 96-well plate?
  • Must I perform paired-end sequencing with NEXTFLEX Unique Dual Index Barcodes?
  • Can I use the NEXTFLEX Unique Dual Index Barcodes in single index experiments?

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