Improve the Number of On-Target Reads

During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX Universal Blockers are designed to prevent this concatemerization of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.

One Set of Blockers for All Applications

The NEXTFLEX Universal Blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI).

Figure 1. NEXTFLEX Universal Blockers (UB) work from 0-19 bp unblocked regions (barcodes and/or UMIs). Competitor A1- and A-3 are two separate products from Competitor A.

Figure 2. NEXTFLEX Universal Blockers can block Illumina TruSeq™ or Nextera™ -style libraries. Competitor A1- and A-2 are two separate products from Competitor A.

The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX or Illumina TruSeq library prep kits, and with any libraries prepared with NEXTFLEX Dual-Index Barcodes or NEXTFLEX Unique Dual Index Barcodes.

They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.