A streamlined approach to quantify inflammatory cytokines from serum and skin

Cytokine release syndrome (CRS) – a systemic inflammatory condition triggered by the activation of immune cells such as NK cells, macrophages, and dendritic cells – is characterized by high levels of inflammatory cytokines and can be triggered by infections causing sepsis or by treatments such as CAR-T cell therapy, among other factors. The resulting feedback loop of intensified inflammation, generally, could also lead to severe tissue damage. Therefore, the ability to consistently quantify local immune mediators is important for research labs looking to understand CRS and how to mitigate its detrimental effect on the body.

In this application note

• Review comparative performance data between a probe sonicator and a bead mill homogenizer on protein analyte levels detected from skin samples
• Access data from serum and skin samples for 13 cytokines measured (13-plex) using the LEGENDplex™ Mouse Cytokine Release Syndrome Panels from an LPS-induced murine model

For research use only. Not for use in diagnostic procedures.