Cytokine Release Syndrome (CRS) can lead to significant tissue damage, and it is an active field of immunology research. We outline a workflow for tissue lysate preparation and quantification of 13 key biomarkers relevant to CRS using the LEGENDplex™ panels and the Omni Bead Ruptor Elite homogenizer. The results show that bead mill homogenization is effective in disrupting tissues for cytokine quantification to support research on inflammatory processes.
For research use only. Not for use in diagnostic procedures.
Quantification of inflammatory cytokines from spleen and liver tissue