DELFIA TRFアッセイ

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody. Alpha technology offers a simple, straight forward workflow. No wash needed! Add sample Add Acceptor bead mix; incubate 1 hr Add Donor beads; incubate 30 mins Read on Alpha-enable microplate reader Download this application note to see how no-wash AlphaLISA ™ technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs), especially at the low concentrations that may be present in hybridoma supernatants. An assay used in these conditions must be sensitive, specific, reliable, reproducible and easy enough to handle 1,000 to 1,500 samples in one batch.

DELFIA immunoassays are a superior alternative to traditional ELISAs

DELFIA TRF technology toolbox reagents and kits for immunodetection. Overcome sample dilution and signal stability challenges encountered with ELISAs via seamless conversion to a fluorescence-based immunoassay technology that delivers greater sensitivity, wider dynamic range, and unmatched signal stability. DELFIA™ TRF, a proven immunoassay technology, parallels protocol similarity to wash-based ELISAs but with minimal steps to enable seamless conversion of current assay workflow. DELFIA (Dissociation-enhanced lanthanide fluorescence immunoassay) TRF (time-resolved fluorescence) technology is a highly-sensitive wash-based immunoassay platform which utilizes a lanthanide chelate to reduce background and improve assay dynamic range. DELFIA offers significant advantages over traditional ELISA, while maintaining similar workflow. High Sensitivity li> Wide dynamic range Prolonged signal stability for detection convenience Proven and validated technology DELFIA Toolbox reagents offer the flexibility to develop custom assays using same ELISA-based or other preferred antibodies, specific to the analyte of interest. Additionally, DELFIA Toolbox Kits contain DELFIA uncoated strip well or ½ Area plates that are high protein binding. Other plates are also available for DELFIA assays. For research use only. Not for use in diagnostic procedures.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD-1/PD-L1 binding inhibitors.

Your specific applications deserve the best microplates! For a researcher, the need to select the most suitable application-specific microplates is critical. Format, color, and surface properties are among the different features that can impact your assay performances and the quality of your results. Covering a vast array of application areas and over many years, Revvity has been committed to offering you high quality plates for the highest quality results. Download this guide and let us walk you through the process of choosing the correct microplate for your specific application.

Discover expert guidelines for optimizing cell labeling in DELFIA TRF cytotoxicity assays. Learn how to enhance assay performance, manage spontaneous release, and achieve accurate results with this comprehensive application note.

Investigate the dynamics of Interactions Kinetic values are of importance for characterizing protein-protein interactions. Studying them brings insight regarding macromolecule binding. Accessing kinetic values is therefore important. This application note dives deep into the topic to provide key guideline and experiments for determining true Kd values in protein-protein interaction assays with HTRF. Benefit from an expert's insight on: Handling HTRF data Determining Kd Investigating Prot-Prot Interactions

This detection method is similar to a traditional ELISA, however DELFIA’s utilization of lanthanide metals endows DELFIA with several distinct advantages over ELISA technology.

We report here the development of a Dissociation Enhanced Lanthanide Fluorescence Immunoassay (DELFIA) for the detection of ADA in serum samples. The use of DELFIA technology in immunogenicity testing was successfully demonstrated in a bridging format with a model antibody system.

Antibody-dependent cellular cytotoxicity (ADCC) is a cell-mediated immune response where an effector cell destroys an antibody-bound target cell. This process involves cells that express tumor or pathogen-derived antigens on their surface, which are targeted by therapeutic antibodies. The binding of the antibody and the effector cell triggers a series of signaling events, leading to the activation of the effector cell and the release of chemokines and cytokines into the tumor microenvironment. Simultaneous detection of ADCC activity and release of chemokines and cytokines from activated NK cells is possible via the combined use of DELFIA TRF and AlphaLISA technologies. Read this application note to access: ADCC assay protocol - ADCC experiment with rituximab Data collection and analysis Formula Calculation and detailed results

Chimeric antigen receptor (CAR) T-cell therapy has transformed the field of immuno-oncology providing a novel approach to treating certain blood cancers. As innovation progresses with the design of next-generation chimeric receptors and a better understanding of the tumor microenvironment, CAR-T therapy has the potential to also revolutionize therapies for treating solid tumors. Whether you are looking to characterize scFv binding capabilities of your chimeric receptor, assess vector transduction efficiency of your CAR construct, measure T-cell proliferation and toxicity, or assess effector cell function of your CAR-T therapy, we have the solutions to fit your needs. Learn more in this flyer about the products designed to support your CAR-T research!

Your dream assay does not exist yet? Create it! This guide will provide you with tools to help you develop your very own protein:protein interaction assays for your research using HTRF™. Detailed steps, microplate plans, optimization procedures and data processing methods: let yourself be guided along the way to creating the assays for your experiments. Features Information relative to reagents, buffers and instrument handling Detailed guidelines and matrices to build, optimize and analyze your own assays Extensive example from our R&D to provide support

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

Asthma is a chronic inflammatory disease that affects millions of people around the world. The detection of specific asthma biomarkers in serum can help diagnose and monitor the disease. AlphaLISA is a bead-based assay technology for the detection of these molecules. This technology was used to quantify biomarker levels in serum samples, specifically Human IL-21, IL-33, and TNFα. Read this application note to: .discover in detail how AlphaLISA technology can be used to quantify biomarker level in serum samples, ensure proper and accurate detection of biomarkers in serum, dilution linearity and spike-and-recovery experiments to determine the proper diluents and dilution factors required for accurate recovery.