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Application Note

Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.

Find out how to optimize cell labeling for DELFIA TRF cytotoxicity assays. This comprehensive application note provides essential tips and techniques to enhance your assay performance, minimize spontaneous release, and achieve accurate results.

  • Introduction to DELFIA EuTDA cytotoxicity reagents kit: overview of the assay principle and its applications in monitoring cytotoxic events
  • Optimization guidelines: detailed instructions on optimizing cell labeling, including factors like BATDA concentration, loading time, and temperature.
  • Experimental methods: step-by-step procedures for cell preparation, labeling, washing, and plating.
  • Data collection and analysis: techniques for measuring DELFIA signals and calculating specific and spontaneous release.
  • Results and discussion: comparative analysis of different cell lines and the effectiveness of inhibitors in improving assay windows.

For research use only. Not for use in diagnostic procedures.

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Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.

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