Biologics Screening and Characterization Assays

Get the best out of your phosphorylation assays Combining phospho and total protein assays enables better analysis. This Application Note provides valuable guidelines for efficiently analyzing and interpreting results from such assay combinations. Check out all the tips and examples in features! Features Introduction to phospho and total protein assay relevance Tips for handling and interpreting data Examples from actual experiments

G-protein coupled receptors (GPCRs) are crucial transmembrane proteins involved in cellular signal transduction. This technical note outlines a method for preparing GPCR membrane model extracts from stable cell lines, specifically for use with the HTRF GTP Gi binding assay. Get this technical note and discover: Key Highlights such as the Importance of GPCRs and the advantages of using HTRF GTP Gi Binding Assay Detailed Method with Cell Culture Preparation, Cell Lysis, Membrane Preparation and Assay Optimization For research use only. Not for use in diagnostic procedures.

The RAS family of genes, particularly K-Ras, plays a critical role in cancer biology. Despite its notorious difficulty as a therapeutic target, recent breakthroughs have brought new hope in treating cancers driven by K-Ras mutations. Our latest application note delves into innovative approaches to K-Ras inhibition, including small molecule inhibitors, synthetic lethality strategies, and PROTAC® molecules. We also showcase the high specificity and sensitivity of the no-wash HTRF™ K- Ras immunoassay, a cutting-edge tool that offers a reliable and precise method for evaluating K-Ras protein levels, outperforming traditional techniques. Discover how this assay can accelerate your research in targeting the elusive K-Ras.

Atherosclerosis pathogenesis, cellular actors, and pathways Atherosclerosis is a common condition in which arteries harden and become narrow due to a build-up of fatty material, usually cholesterol, and other substances such as calcium. This can lead to a range of serious health complications, including heart attack or stroke, making the disease an important contributing factor in death and morbidity in developed countries. Recent developments in our understanding of atherosclerosis from a molecular perspective include the discovery of new players in disease pathogenesis. Included in this white paper Atherosclerosis: step-by-step pathogenesis, therapeutic strategies, and recent developments Detailed descriptions and explanations, including a focus on pathways

Compound MoA and potential cytotoxic effects deciphered thanks to immunoassays and cell viability assay The attrition of drug molecules occurs at various stages in the development process, and most early-phase failures are attributed to safety and toxicity issues. Considering the economic impact of early project termination, the biggest challenge comes from selecting the most potent and selective drug compounds while assessing their potential toxic side effects. In this application note, you’ll discover how to easily combine AlphaLISA™, HTRF™, or AlphaLISA™ SureFire® Ultra™ immunoassays with the ATPlite™ 1step cell viability assay to simultaneously, and in a single sample, study the efficacy of drug compounds on disease pathways while identifying possible cytotoxic effects. Recommendations Detailed workflows Case studies on various cell lines and different markers using HTRF, AlphaLISA, or AlphaLISA SureFire Ultra immunoassays together with the ATPlite 1step cell viability assay

Get a useful overview of today’s immunity knowledge with this booklet Immunity is a collection of complex processes involving multiple strategies and specialized cell types. This booklet provides you with critical information regarding their roles, characteristic and signaling pathways as well as the collaborative behaviors that contribute to immunity. Featured in this guide: Review the fundamentals of immune cell types and mechanisms Learn from a cutting-edge research report Pathways and functional details on over 10 specialized immune cells

Dive deeper into research on the GPCR signaling pathway β-arrestins are intracellular proteins that play an important role in GPCR signaling. Complexes formed between ligand-occupied GPCRs and β-arrestin lead to interaction with adaptor protein AP2. This interaction is followed by internalization of the receptors. HTRF technology is effective for studying the interaction between AP2 and β-arrestin2. Get your application note to discover: The applicability of the β-arr2 recruitment kit to a variety of compounds acting on β-arrestin2, and its ability to correctly rank pharmacological compounds (agonists and antagonists) How you can detect the β-arrestin2 / AP2 interaction for all classes of GPCRs Detailed experimental conditions and explanatory diagrams

The definitive guide to setting up a successful cytokine assay Many therapeutic areas require an understanding of cytokine release. When preparing for a cytokine assay, many underappreciated parameters (e.g. sample handling, cell culture format, sample dilutions) can in fact greatly impact the performance of the cytokine detection. This guide reviews the latest knowledge surrounding the proper use of HTRF cytokine assays. A review of the key terms and definitions in cytokine detection A list of optimization steps Recommendations for data analysis

Ask real blood for real responses Fresh blood is the model of choice to study drug immunotoxicity and predict adverse effects. Written in collaboration with Blood Assay Solutions, this note provides guidelines for fresh blood cytokine quantification. Discover the power of our cytokine portfolio for your research. Features Step by step protocols for fresh blood cytokine quantification in your research Examples of pathway stimulation assays (TCR, TLR …) Comparison beteen fresh blood and PBMCs

Evaluation of the therapeutic profile of anti-oncogene compounds in various cell lines with AlphaLISA™ and HTRF™ KRAS is a proto-oncogene known to be mutated in many cancer subtypes, inducing uncontrolled proliferation and cell metabolism changes. Like most small GTPases, KRAS will bind to GDP in its inactive form or to GTP in its active form. KRAS G12C is one of the most commonly found mutant forms in cancers, and leads to a permanently active state of KRAS. The upregulation of KRAS interaction with the exchange factor SOS1 leads to cancer phenotypes. Reducing KRAS activity and associated pathways could control the biological processes involved in cancer growth. Furthermore, it is well known that KRAS induces activation of mitogen-activated protein kinase (MAPK), thus playing a central role in human cancers. This application note provides a convincing demonstration of the reliability of the AlphaLISA and HTRF KRAS portfolios to evaluate compound in vitro therapeutic profiles in a cellular context: Determine the effects of KRAS and SOS1 inhibitors in different human cancer cell lines Discriminate the cellular action of KRAS-targeting compounds and evaluate their effectiveness in modulating KRAS downstream pathways.

Challenge the limits of binding kinetics studies This Note describes how binding kinetics studies can be enriched with a K on , K off approach by providing critical data on how the association and dissociation rates of a receptor-ligand couple can be assessed thanks to streamlined, no wash Tag-lite assays. Learn how to process and analyze the data, and discover how receptor binding kinetics offers significant insights into your compound’s mode of action. Features: Materials and methods for the experiment Data processing and result analysis Examples from our R&D

The essential guide to Gq signaling This White Paper aims to provide you with the information related to the Inositol phosphate approach for Gαq signaling studies. Technology principle, ease of use, performance, specificity and more! All these topics are reviewed in this comprehensive guide. Review the fundamentals of Inositol signaling Avoid the pitfalls of hemi-equilibrium Make the most of unmatched specificity

Get a picture of today’s Kinase knowledge Kinases are involved in many diseases, including cancer, cardiovascular disease, and neurological disorders. Developed in collaboration with Labiotech, this white paper provides you with critical information about how kinases influence disease and helps you learn more about targeted kinase therapies. Features: Review the fundamentals of Kinase research Learn from a cutting-edge content

Dive deeper into NASH investigation. Nuclear Receptors (NRs) regulate gene transcription by DNA binding. Interaction of NRs with an agonist triggers conformational changes and enables the recruitment of coactivators (CoA), leading to gene transcription. The activity of several NRs has been associated to NAFLD, bringing about the emergence of new therapeutic targets in NASH. Now, thanks to HTRF technology, you can monitor NR/CoA recruitment easily. With this application note you will: Benefit from HTRF technology in your research to identify potent NR ligands Discover the potential of HTRF PPi reagents to revolutionize your research Build robust and convenient NR/CoA recruitment assays for your research

Discover a rapid and reliable tool for the identification and characterization of STING-targeting compounds. Quantifying human IFN-β using an HTRF-based approach This note demonstrates how using HTRF to quantify human IFN-β is a powerful and effective method for the characterization of STING agonists. It provides data demonstrating that the quantification of IFN-β using HTRF is well correlated with ELISA assays. This note also provides data about gene reporter assay correlation with our HTRF IFN-β assay.

Discover the benefits of using HTRF assays to characterize liver fibrosis. Several molecular markers have been investigated as possible factors in the progression of non-alcoholic steatohepatitis (NASH) towards liver fibrosis. The TGF-b/SMAD/α-SMA pathway has a crucial role in this progression and has emerged as an important therapeutic target. This application note will allow you to: Get an overview of the liver fibrosis mechanism’s function and dynamics Take advantage of HTRF technology to study the TGF-β/SMAD/α-SMA signaling axis Benefit from additional content to help you revolutionize NASH discovery

Discover the cutting edge of kinase assay knowledge This literature review illustrates the possibilities and versatility of HTRF™ KinEase assays in plural contexts. It features experiments and data from published studies that investigated compound action on kinase activity or helped to kinase characterization. It features: Introduction to HTRF KinEase assays Recent results on various therapeutic areas

A Bruton Tyrosine Kinase (BTK) case study PROteolysis Targeting Chimeras (PROTACs) are one of the latest trending tools used in drug discovery. This note compiles experiments carried out to characterize PROTAC compounds using Bruton Tyrosine Kinase (BTK) as a model on Ramos cells. Both AlphaLISA and HTRF technologies were used in biochemical and cell-based contexts. Featured in this note: Assay principle and workflow Consistent results (data and graphs) Informative materials and methods, including references

Dive deeper into fibrosis research In fibrotic disorders, the normal regulation of the extracellular matrix (ECM) is compromised, which leads to excess of ECM or lack of MMPs. This can further lead to a disorganized accumulation of ECM which can ultimately result in organ rigidity and fibrosis. For this reason, the ECM constitutive proteins, MMPs and fibroblasts activating and regulatory mediators such as TGF-β1 and PGE2 are hot spots of therapeutic research for fibrosis. With this review, you will: Better understand how COX enzymes regulate PGE2 levels in TLR stimulated fibroblasts Learn how an AlphaLISA protein-protein interaction assay can be used as a screening platform for TG2-Fibronectin inhibitor identification Discover how CKD might impact the malignant myocardial fibrosis factor combination

A comprehensive overview of fibrosis development Fibrosis is a main contributor to a wide range of organ failures which stems from a mis-regulation of inflammation in response to injury. Fibrosis occurs when the long-term remodeling and removal of ECM suffers from a higher production than removal rate. This event is associated with chronic inflammation, which keeps myofibroblasts active for extended periods, thus making them and their ECM protein secretion the main effectors of pathological fibrosis. This guide is intended to help scientists and researchers appreciate and navigate the cellular and signaling actors of the development of fibrotic pathologies and disorders. Review the fundamentals of fibrosis as a process. A look at TGF-β1 signaling pathways as therapeutic approaches for fibrotic disorders. Insight into immune cell types involved and treating and reversing progressive.

Helping you select an optimal assay for your research Revvity offers a comprehensive line of insulin quantification assays designed to significantly reduce the time to results, sample consumption, and cost of quantifying insulin for your research. This brochure describes the functionality and benefits of all 3 assay kits available for insulin quantification.

A single White Paper for a review of immuno-oncology Immuno-oncology, the field which stimulates a individual's own immunity in order to fight cancer, has seen major advances since the 19th century and is now one of the most promising approaches against cancer. This White Paper provides you with relevant information related to: Immuno-oncology history Passive therapies - review Active therapies - review Immuno-oncology’s future

PBMCs: Waste no more There is no reason to waste precious, hard-to-obtain unused cells! This Technical Note shares our internal protocol to freeze and thaw PBMCs to obtain cells every time you need them. These guidelines are a great companion to our Technical Note, “Guidelines for PBMC isolation.” Don’t throw away your cells! Features: PBMC freeze/thaw protocol Validation data

More interactions within range This Technical Note wants to help you develop the PPI assay of your dreams using HTRF™. As well as an introduction to the technology, it features the essential guidelines, with detailed instructions and microplate matrices, to develop, analyze, optimize and finally perform the assays you wish for. Features: Introduction to HTRF assays and tips on experimental conditions Step by step instructions and microplate matrices for each stage in development and optimization Examples for proper data analysis