Maternal Cell Contamination Test -MCC

Test Details Page

Collection	Required DNA Quantity by Test Type*: Next Generation Sequencing (NGS): Send >1000 ng total gDNA @ >15 ng/μL. Please ship samples in 10mM Tris. Do not use EDTA. Sanger Sequencing: Send >500 ng total gDNA @ >15 ng/μL (varies by the size of the gene and the variants requested). Non-Sanger Sequencing Tests: Send >500 ng total gDNA @ >15 ng/μL.
Sample Condition	* Required DNA Quality: High molecular weight DNA (>12kb). A260/A280 reading should be ≥ 1.8. A260/230 a ratio range of 1.8 to 2.2. Contact the laboratory for specific amounts if total ng cannot be met.
Shipping	Ship overnight at ambient temperature.
SPECIAL SAMPLE INSTRUCTIONS	Research Laboratories: DNA extracted in research laboratories is not acceptable. Only under exceptional circumstances (e.g., proband not available) will DNA extracted in a research laboratory be accepted for clinical testing. Additional testing (e.g., of other family members) may be required to confirm results. Laboratories outside the United States: Non-US laboratories are not subject to CLIA regulations and will be reviewed on a case-by-case basis. Please call to speak with a laboratory genetic counselor before submitting a DNA sample from any non-CLIA-certified laboratory. Special Notes: If extracted DNA is submitted, information regarding the method used for extraction should be sent along with the sample.

Test Details Page

Collection Container(s)	Sterile, screw-top container filled with tissue culture transport medium.
Collection	All prenatal specimens will be tested for maternal cell contamination (MCC). Send maternal blood (EDTA tube) for comparison. Transport products of conception and/or fresh tissue (minimum: 10 mg) in a sterile, screw-top container filled with tissue transport medium. Do not place in formalin. If the specimen is too large for a normal collection tube, a larger sterile container can be used.
Sample Condition	If the autopsy is performed: Facia lata, diaphragm, tendon, skin, tissue from internal organs (if fresh), chest wall cartilage (particularly if macerated), or placenta from fetal side. If no autopsy is performed: Placenta from fetal side is preferred, e.g., villi. Umbilical cord is also acceptable. State clearly in the TRF whether the tissue was of fetal or placental in origin.
Shipping	Shipped immediately at ambient temperature or with a cold pack, especially under hot weather to the laboratory by overnight express with arrival Monday-Friday only. Alternatively, fresh POC and/or tissue samples can be snap-frozen and kept at -80°C. Frozen tissue samples should be shipped overnight on dry ice with arrival Monday-Friday only - NO WEEKEND SHIPMENTS!
SPECIAL SAMPLE INSTRUCTIONS	For prenatal samples, Revvity Omics will provide a shipping label to use for shipping the sample to our lab. Please call 1 (866) 354-2910 to arrange this. At this time, you will also be connected to a laboratory genetic counselor to answer any questions about the testing. For samples originating outside of the United States, please contact your nearest Revvity Omics laboratory for more information.

Test Details Page

Collection Container(s)	EDTA (Purple Top) or ACD (Yellow Top)
SPECIAL SAMPLE INSTRUCTIONS	Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types.

Test Details Page

SPECIAL SAMPLE INSTRUCTIONS	Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types.

Test Details Page

SPECIAL SAMPLE INSTRUCTIONS	Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types.

Test Details Page

Collection Container(s)	EDTA (purple top)
Collection	Infants (< 2-years): 2 to 3 mL; Children (>2-years): 3 to 5 mL; Older children and adults: Minimum 5mL. The blood tube should be inverted several times immediately after blood collection to prevent coagulation.
Sample Condition	Store at ambient temperature. Do not refrigerate or freeze.
Shipping	Ship overnight at ambient temperature ensuring receipt within 5-days of collection.
SPECIAL SAMPLE INSTRUCTIONS	Clotted or hemolyzed samples are not accepted.

Test Details Page

SPECIAL SAMPLE INSTRUCTIONS	Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types.

Test Details Page

Collection Container(s)	EDTA (Purple Top) or ACD (Yellow Top)
Collection	Collect 1 to 3 mL of fetal blood.
Sample Condition	Store at ambient temperature. Do not refrigerate or freeze.
Shipping	Ship overnight at ambient temperature ensuring receipt within 5 days of collection.

Test Details Page

SPECIAL SAMPLE INSTRUCTIONS	Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types.