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Technical Note
Kinetic analysis of bioluminescent sources for in vivo imaging
Technical highlighting kinetic analysis of bioluminescent sources for in vivo imaging
Technical Note
How to determine saturation in your in vivo optical imaging studies
This technical note outlines the prodecures for determining saturation in you in vivo optical imaging studies using the IVIS platform.
Technical Note
Working with 2D image overlay using Living Image Software
This technical note outlines loading multiple images and overlaying them, for example bioluminescent tumor with fluorescent targeted drug acquired in two separate images
Technical Note
Fluorescence tomography - Setup and sequence acquisition
This tech note will discuss how to set up an imaging experiment in Living Image for fluorescence tomography using IVIS imaging systems.
Technical Note
Transillumination fluorescence using Living Image software
This tech note will walk you through the steps of manually entering your settings for a transillumination sequence. The Living Image software include an Autoexposure setting and an Imaging Wizard and it is recommended that you use these features to simplify the setup.
Technical Note
Transillumination fluorescence - raster scan
The tech note/guide discusses raster scanning within Living Image software to faciliate faster transillumination imaging
Technical Note
Trans-illumination fluorescence - normalized transmission fluorescence
The tech note/guide discusses normalized transmission fluorescence is a technique that allows us to subtract background light leakage through thin tissue from transillumination images utilizing an extra image captured with a neutral density (ND) filter
Technical Note
Subtracting background ROI from the measurement ROIs when performing fluorescence quantitative analysis
This tech note discusses why it's recommended to subtract background region of interest (ROI) from the measurement ROIs when performing fluorescence quantitative analysis.
Technical Note
Working with Image math to subtract background from images without performing spectral unmixing
This tech note highlights working with Image math to subtract background from images without performing spectral unmixing
Technical Note
Working with 3D image overlay using Living Image Software
This tech note describes using Living Image software to copy 3D sources (voxels) from one 3D reconstruction into another
Technical Note
Bioluminescence tomography - source reconstruction and analysis
Technical note highlighting bioluminscent source reconstruction and analysis on the IVIS Spectrum platform
Application Note
Understanding the anti-inflammatory effects of a microbiota-derived metabolite using high-content analysis and AlphaLISA
Here we combine HCA and AlphaLISA cytokine detection to further characterize the anti-inflammatory effect of UroA on LPS-stimulated human macrophage-like cells.
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