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Publication Icon   Literature - Publication Review
HTRF and AlphaLISA assays bring clarity to extracellular matrix related fibrosis development
This note gathers publications that exemplify various applications of HTRF™ and AlphaLISA™ assays to advance fibrosis related research and shows how other researchers have harnessed these technologies to move their studies forward.
Whitepaper Icon   Whitepaper
Alpha technologies for antibody detection and characterization
Alpha technology is homogeneous and non-radiometric with distinct features that makes it enabling in comparison to other proximity assays.
Whitepaper Icon   Whitepaper
Sugar with your protein? - Generation of a fucosylation-deficient CHO cell host to produce therapeutics with enhanced potency
Detailed overview of how CHO cells have been genetically modified to enable production of afucosylated antibodies with enhanced ADCC activity for improved biotherapeutics
Application Note Icon   Application Note
AlphaLISA human IL-6 kit can quantify pyrogens in monocyte activation tests
Usefulness of the AlphaLISA IL-6 kit for the quantification of pyrogens and the ease of use of a homogeneous, wash-free assay with low sample volumes.
Application Note Icon   Application Note
Evaluating the specificity of PD-1 and PD-L1 blocking antibodies using AlphaLISA human and mouse PD-1/PD-L1 binding kits
Find out how the AlphaLISA human/mouse PD-1/PD-L1 binding assays provide a fast, powerful, homogeneous platform for obtain binding potencies from potential novel drug candidates.
Application Note Icon   Application Note
FcRn binding assays using AlphaLISA and HTRF technologies
In this application note you will find information about how both AlphaLISA™ and HTRF™ assays can help to measure the relative affinities of therapeutic antibodies to FcRn.
Infographic Icon   Infographic
Ditch the wash steps and accelerate discovery
ELISA immunoassays are great, but what if you could improve sensitivity and increase dynamic range all while eliminating wash steps and saving time?
Application Note Icon   Application Note
Immunogenicity assessment using the AlphaLISA technology
In this application note, we demonstrate that the AlphaLISA™ mix-and-read homogeneous assay permits the sensitive detection of anti-drug antibodies (ADA). Learn more.
Publication Icon   Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments
This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.
Application Note Icon   Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note Icon   Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Guide Icon   Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
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