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Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to the combination of AlphaLISA™, HTRF™ or AlphaLISA™ SureFire® Ultra™ immunoassays with ATPlite™ 1step cell viability assay.
Whitepaper
Addressing the challenges of bispecific antibody characterization with high-throughput platforms
With the complexities of bispecific antibody development and characterization explore the binding assays and technology through real-world examples
Application Note
Rapid, no-wash measurement of immune checkpoint molecules and cytokines in co-cultures of immune cells and cancer cell lines
Download this application note to learn how to rapidly measure multiple biomarkers in cell culture supernatant and lysates
Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
This application note shows that AlphaLISA can be used to compare differences in protein expression levels and cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.
Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
Application Note
Immunogenicity assessment using the AlphaLISA technology
In this application note, we demonstrate that the AlphaLISA™ mix-and-read homogeneous assay permits the sensitive detection of anti-drug antibodies (ADA). Learn more.
Application Note
Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits
This application note demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigenstimulated PBMCs isolated from cow blood and shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.
Application Note
FcRn binding assays using AlphaLISA and HTRF technologies
In this application note you will find information about how both AlphaLISA™ and HTRF™ assays can help to measure the relative affinities of therapeutic antibodies to FcRn.
Application Note
Monitoring residual HEK293 DNA in AAV-based therapies using the HostDetect HEK293 PCR DNA quant kit
Data behind the development of the HostDetect Hek293 kit that offers a PCR-based approach for quantifying residual host cell DNA
Whitepaper
Learning from our ancestors: can we be better prepared for the next pandemic?
Researchers have found evidence of an ancient viral epidemic involving host coronavirus interacting genes in East Asia more than 20,000 years ago. These findings could help countries prepare for future pandemics.
Application Note
Avoiding biotin interference in AlphaLISA assays
This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A
In this application note we demonstrate the ease with which AlphaLISA toolbox reagents can be used to develop any FcγR binding assay across the various stages of biologics research and development, including therapeutic screening and GMP Lot Release.
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