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Technical Note
Lysis buffer compatibility HTRF assays
This leaflet presents the lysis buffer compatibility for HTRF assays.
Technical Note
TRF assay performance of the EnVision Nexus multimode plate reader
This technical note highlights the capabilities of the EnVision Nexus™ in delivering efficient results for HTRFTM and LANCETM immunoassays, establishing it as a leading system in the next generation of plate readers.
Technical Note
Insider tips to optimize your GPCR signaling studies
This technical note provides valuable tips for performing Advanced phospho-ERK1/2 assays on adherent cell lines expressing GPCRs and will ensure optimal results.
Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
HTRF setup recommendations for EnVision
This document outlines setup options for properly configuring the EnVision multimode plate reader to achieve optimal HTRFTM readout.
Technical Note
Evaluating protein phosphorylation and cellular signaling cascades within the integrated stress response pathway
Protein phosphorylation is vital in cellular processes. AlphaLISA™ SureFire® Ultra™ assays accurately measure phosphorylation events, aiding compound activity study.
Technical Note
Comparison of cell concentration and viability using the Cellometer Auto 2000 cell counter and a manual hemocytometer.
Technical note comparing measurement of cell concentration and viability using the Cellometer Auto 2000 cell counter and a manual hemocytometer.
Technical Note
Biotin-free AlphaLISA assays for the detection of cytokines in PBMC supernatants
In this technical note, AlphaLISA biotin-free kits were used to detect and quantify the cytokines interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6).
Technical Note
Expanding imaging multiplexing capabilities with PhenoVue Fluor 400LS - Phalloidin
This technical note offers tips and best practices relating to fluorescent signal quality using PhenoVue Fluor 400LS Phalloidin stain.
Technical Note
Quantifying TNFR1 in both soluble and membrane bound form using AlphaLISA technology
Quantifying TNFR1 in both soluble and membrane bound form using AlphaLISA technology
Technical Note
Quantitative analysis of lysine 36 demethylation with AlphaLISA
This technical note provides details on how the AlphaLISA immunodetection assay quantifies the demethylation of a biotinylated Histone H3 (21-44) peptide that is tri-methylated at lysine 36
Technical Note
AlphaLISA Acetyl‑Histone H3 lysine 9 (H3K9ac) cellular assay
This technical note provides information on how the AlphaLISA immunodetection assay detects variations in acetylated histone H3 lysine 9 (H3K9ac) levels within cellular extracts
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