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Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
AlphaLISA DOT1L histone H3 lysine-N-methyltransferase assay
This Technical Note will provide you with guidelines on the optimization of a DOT1L enzymatic assay using oligonucleosomes as the substrate
Technical Note
The AlphaLISA™ Approach to TNFR1 Measurement: Insights from Human Urine Samples
Detailed in this technical document is the method for assessing TNFR1 concentrations in human urine, showcasing a comparative analysis between samples from healthy individuals and those with type 2 diabetes.
Technical Note
AlphaLISA JMJD3 Histone H3-Lysine 27 demethylase assay
This AlphaLISA immunodetection assay measures the demethylation of a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 27.
Technical Note
AlphaLISA in urine: Detecting kidney injury marker KIM-1
This document highlights the capability of AlphaLISA technology to identify the kidney injury biomarker KIM-1 in urine samples, achieving detection levels as low as 14 pg/mL.
Technical Note
The Advantages of AlphaLISA™ Technology: A Study with Human TNFR1
This technical note compares ELISA kits from two suppliers to the Human TNFR1 AlphaLISA™ Detection Kit to demonstrate the ease of use and benefits of Alpha technology.
Technical Note
AlphaLISA SET7/9 Histone H3-lysine N-methyltransferase assay.
this AlphaLISA immunoassay detects the mono-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 4.
Technical Note
AlphaLISA PRMT6 Histone H3-Arginine N-methyltransferase assay
This AlphaLISA immunoassay detects the mono-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 4.
Technical Note
AlphaLISA SIRT1 p53 lysine 382 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated p53 (368-393) peptide acetylated at lysine 382.
Technical Note
AlphaLISA HDAC1 Histone H3-Lysine 27 deacetylase assay
In this technical note discover how The AlphaLISA assay detects detect the removal of acetyl groups from a biotin-tagged peptide derived from Histone H3.
Technical Note
Exposing and detecting membrane and intracellular proteins: AlphaLISA simplifies the detection of HO-1
This technical document reveals the application of AlphaLISA technology in measuring HO-1 concentrations within HeLa cell extracts.
Technical Note
AlphaLISA SIRT1 Histone H3-Lysine 4 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4.
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