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Application Note
Enhance PROTAC Drug Discovery with AlphaLISA and HTRF Kits
This note compiles experiments carried out to characterize PROTAC compounds using Bruton Tyrosine Kinase (BTK) as a model on Ramos cells.
Application Note
Detection of MAPK activation to evaluate the efficacy and potency of KRAS/SOS1 inhibitors by AlphaLISA and HTRF technologies
Detection of MAPK activation to evaluate the efficacy and potency of KRAS/SOS1 inhibitors by AlphaLISA and HTRF technologies
Application Note
Using IP1 assays to study FGFR mediated pathways for cancer drug research
Detection of IP1 accumulation was achieved using either a no wash Revvity HTRF or AlphaLISA kits. These assays enable the rapid and accurate measurement of the activation status of intracellular downstream key enzymes and second messengers
Application Note
Optimizing ternary complex assays with AlphaLISA Toolbox reagents for targeted protein degradation applications
In this application note, discover how easily Revvity AlphaLISA toolbox reagents are used to detect ternary complex with variable ligases, protein partners, and PROTAC molecules.
Application Note
Development of AlphaLISA technology assays for the detection of neutralizing antibodies in human serum
We report here the development of two AlphaLISA functional assays, a cell-based and a biochemical assay, for the detection of neutralizing ADA in human serum samples.
Application Note
Rapid no wash assays for characterizing a mouse TIGIT/PD-L1 bispecific antibody
In this application note, we demonstrate how AlphaLISA™ assay technology can be used for bispecific antibody detection.
Application Note
A comparative study of two immunoassay platforms to determine lentivirus titer for CAR-T development
This application note demonstrates a comparative quantification of the p24 titer in a lentiviral GFP control sample using Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms.
Application Note
Development of pharmacokinetic (PK) assays for detecting biosimilars targeting TNF alpha using AlphaLISA
In this Application Note, AlphaLISA™ assays are used for detecting biosimilars targeting TNF alpha. Learn more.
Application Note
Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits
This application note demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigenstimulated PBMCs isolated from cow blood and shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.
Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to the combination of AlphaLISA™, HTRF™ or AlphaLISA™ SureFire® Ultra™ immunoassays with ATPlite™ 1step cell viability assay.
Application Note
Utilization of AlphaLISA technology to accurately detect asthma biomarkers in human serum
In this application note, AlphaLISA technology was used to quantify biomarker levels in serum samples, specifically Human IL-21, IL-33, and TNFα.
Application Note
Fc-Receptor binding ADCC assays utilizing AlphaLISA technology: Characterization of hIgGs and FcγRIIIa
This application note demonstrates how AlphaLISA assay can be used to determine the binding activity of human IgG Fc fragment to human FcγRIIIa and to study how other antibodies bind to FcγRIIIa.
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