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Application Note
Determination of association and dissociation rates constants using the Tag-lite platform
The flexibility and throughput of the Tag-lite platform enables fast and easy calculation of the koff and kon constants for a given receptor.
Application Note
Assessment of drug efficacy and toxicity by combining innovative technologies
Learn how to simultaneously detect drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite.
Application Note
A guideline for HTRF cell-based phospho-protein data normalization
This application note illustrates how to calculate a normalization value and interpret the results correctly when using HTRF phosphospecific and total protein assays.
Application Note
Unlock your STING polymorphism research with a comprehensive HTRF binding platform
This application note demonstrates how the HTRF STING binding assay platform represents a powerful new tool to identify and characterize compounds which bind to the STING protein
Application Note
Sensitive detection of GPCR activation in various cellular models, based on ERK1/2 phosphorylation
This application note demonstrates how the Advanced Phospho-ERK1/2 (Thr202/ Tyr204) kit is suitable for ERK signaling pathway investigations mediated by GPCR stimulation with pharmacological compounds.
Application Note
Pharmacological validation of a panel of GPCRs with the HTRF GTP Gi Binding Assay
This application note discusses the characterization process for candidate molecules targeting Gα-protein activation through GPCRs with the functional HTRF GTP binding assay.
Application Note
AAV capsid detection and characterization with new AlphaLISA assay kits
Revvity has developed and manufactured a line of immunoassays that detect and quantify AAV capsid with AlphaLISA™ technology.
Application Note
Best practices for analyzing tumor xenografts
This technical note provides procedures and guidelines for performing HTRF phospho-protein assays on tumor xenografts, with a particular focus on the preparation of tumor lysates and working samples.
Application Note
HTRF platform for anticancer drug discovery: from 2D and 3D cell cultures to xenograft models
Learn how to analyze cell signaling pathways on in vitro and in vivo sample types routinely used in the anticancer drug discovery process.
Application Note
HTRF reveal subtle effects in tumor xenografts: a method of choice beyond Western blot
Learn about a faster, simpler, and more accurate technique for assessing protein phosphorylation and for revealing subtle drug-induced modulations compared to Western blotting.
Application Note
Download your protocol on CD4 T cells differentiation into Th17 cells
In this application note is reported a protocol for the isolation of mouse naïve CD4+ T-cells and their differentiation into T-Reg and Th-17 cells.
Application Note
Mitigate index hopping and spread of signal on patterned flow cells.
This application note describes the benefits of using UDI Barcodes.
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