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Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
Application Note
Streamline the HEK 293 HCP impurity quantification workflow with new, no-wash AlphaLISA HEK 293 Host Cell Protein Kit
Read our new HEK HCP application note to learn about HEK 293 HCP impurity quantification using AlphaLISA technology (link not available yet)
Flyer
Download your ATPlite flyer
Revvity offers ATPlite™ and ATPlite 1step assays to measure cellular ATP levels, indicating cell viability and proliferation, using firefly luciferase and D-luciferin. Check out our flyer for the latest solutions in cytotoxicity, cell viability, and cell proliferation assays.
Application Note
Alpha technology: a fast and sensitive orthogonal approach to cell-based potency assays
Learn why Alpha technology is an ideal cell-free orthogonal system for potency assays.
Literature - Publication Review
HTRF and AlphaLISA assays bring clarity to extracellular matrix related fibrosis development
This note gathers publications that exemplify various applications of HTRF™ and AlphaLISA™ assays to advance fibrosis related research and shows how other researchers have harnessed these technologies to move their studies forward.
Technical Note
AlphaLISA Acetyl‑Histone H3 lysine 27 (H3K27ac) cellular detection kit
This AlphaLISA immunodetection assay detects alterations in acetylated histone H3 lysine 27 (H3K27ac) levels within cellular extracts.
Application Note
cAMP assay provides flexibility and stable pharmacology
AlphaScreen™ technology provides a quantitative detection of molecules in a homogeneous, no-wash format and can be applied to GPCR research.
Application Note
A comparative study of two immunoassay platforms to determine lentivirus titer for CAR-T development
This application note demonstrates a comparative quantification of the p24 titer in a lentiviral GFP control sample using Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms.
Application Note
Tracking inflammasome activity via measuring IL-1β levels with AlphaLISA and HTRF
This application note demonstrates the utility of AlphaLISA™ and HTRF™ for the detection of IL-1β in cell supernatant and serum samples which can be used as a biomarker for inflammasome activity
Application Note
cAMP AlphaScreen assay: a method for the pharmacological characterization and screening of Gαi coupled receptors in whole cells
The note demonstrates the utility of the AlphaScreen-based cAMP Assay kit for the pharmacological characterization and potential high-throughput screening of Gαi-coupled receptors.
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