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Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note
Performing AlphaLISA assays with RPMI-1640
This application note provides alternatives for working with samples in RPMI medium, which contains high levels of biotin
Application Note
Fc-Receptor binding ADCC assays utilizing AlphaLISA technology: Characterization of hIgGs and FcγRIIIa
This application note demonstrates how AlphaLISA assay can be used to determine the binding activity of human IgG Fc fragment to human FcγRIIIa and to study how other antibodies bind to FcγRIIIa.
Infographic
Ditch the wash steps and accelerate discovery
ELISA immunoassays are great, but what if you could improve sensitivity and increase dynamic range all while eliminating wash steps and saving time?
Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments
This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.
Application Note
LabChip GXII Touch small RNA assay
Improve the productively and cost effectiveness of high-throughput QC screening of small RNA molecules for genomic research and biotherapeutic development.
Whitepaper
Exploring current and future approaches for targeting KRAS
Get the latest insights on KRAS and see how it has switched from being an undruggable to a druggable cancer target
Application Note
Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.
Discover expert guidelines for optimizing cell labeling in DELFIA TRF cytotoxicity assays. Learn how to enhance assay performance, manage spontaneous release, and achieve accurate results with this comprehensive application note.
Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
Application Note
Immunogenicity assessment using the AlphaLISA technology
In this application note, we demonstrate that the AlphaLISA™ mix-and-read homogeneous assay permits the sensitive detection of anti-drug antibodies (ADA). Learn more.
Application Note
Utilization of AlphaLISA technology to accurately detect asthma biomarkers in human serum
In this application note, AlphaLISA technology was used to quantify biomarker levels in serum samples, specifically Human IL-21, IL-33, and TNFα.
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