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Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Brochure
Drug discovery and development tailor made
Learn more about our Custom Discovery Reagent and Assay Services to further your research, backed by 30+ years of experience.
Technical Note
Development of an AlphaLISA assay to measure and screen inhibitors of the p53-MDM2 interaction
Development of an AlphaLISA Assay to Measure and Screen Inhibitors of the p53-MDM2 Interaction
Application Note
LabChip GXII Touch small RNA assay
Improve the productively and cost effectiveness of high-throughput QC screening of small RNA molecules for genomic research and biotherapeutic development.
Application Note
Performing AlphaLISA assays with RPMI-1640
This application note provides alternatives for working with samples in RPMI medium, which contains high levels of biotin
Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments
This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.
Infographic
Ditch the wash steps and accelerate discovery
ELISA immunoassays are great, but what if you could improve sensitivity and increase dynamic range all while eliminating wash steps and saving time?
Application Note
Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.
Discover expert guidelines for optimizing cell labeling in DELFIA TRF cytotoxicity assays. Learn how to enhance assay performance, manage spontaneous release, and achieve accurate results with this comprehensive application note.
Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
Application Note
Avoiding biotin interference in AlphaLISA assays
This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.
Brochure
GPCRs: the pathway to discovery
Understanding GPCRs and their therapeutic potential can help researchers identify new drug targets.
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