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Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Guide
ELISA to AlphaLISA: Immunoassay conversion made easy
Discover streamlined immunoassays with AlphaLISA™. Convert ELISA to AlphaLISA for precision, proven technology, and versatile measurements.
Guide
Start-up guide: Alpha technology/protein: protein interactions
This quick start guide will help you set-up no-wash AlphaLISA assays for measuring protein:protein interactions.
Technical Note
The AlphaLISA™ Approach to TNFR1 Measurement: Insights from Human Urine Samples
Detailed in this technical document is the method for assessing TNFR1 concentrations in human urine, showcasing a comparative analysis between samples from healthy individuals and those with type 2 diabetes.
Technical Note
The Advantages of AlphaLISA™ Technology: A Study with Human TNFR1
This technical note compares ELISA kits from two suppliers to the Human TNFR1 AlphaLISA™ Detection Kit to demonstrate the ease of use and benefits of Alpha technology.
Technical Note
AlphaLISA SIRT1 p53 lysine 382 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated p53 (368-393) peptide acetylated at lysine 382.
Technical Note
Fast, simple, homogeneous assays for characterization of Fc receptor binding using AlphaLISA
In this technical note discover how the AlphaLISA human FcRn binding kit detects binding between FcRn (neonatal Fc receptor) and human IgG.
Guide
LANCE Ultra cAMP Assay development guidelines
The LANCE™ Ultra cAMP Assay Development Guidelines offer detailed instructions for accurately measuring cyclic adenosine monophosphate (cAMP) levels in cell lysates and membrane samples.
Application Note
LabChip GXII Touch small RNA assay
Improve the productively and cost effectiveness of high-throughput QC screening of small RNA molecules for genomic research and biotherapeutic development.
Application Note
Performing AlphaLISA assays with RPMI-1640
This application note provides alternatives for working with samples in RPMI medium, which contains high levels of biotin
Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments
This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.
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