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Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
The AlphaLISA™ Approach to TNFR1 Measurement: Insights from Human Urine Samples
Detailed in this technical document is the method for assessing TNFR1 concentrations in human urine, showcasing a comparative analysis between samples from healthy individuals and those with type 2 diabetes.
Technical Note
AlphaLISA JMJD3 Histone H3-Lysine 27 demethylase assay
This AlphaLISA immunodetection assay measures the demethylation of a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 27.
Technical Note
The Advantages of AlphaLISA™ Technology: A Study with Human TNFR1
This technical note compares ELISA kits from two suppliers to the Human TNFR1 AlphaLISA™ Detection Kit to demonstrate the ease of use and benefits of Alpha technology.
Technical Note
AlphaLISA SET7/9 Histone H3-lysine N-methyltransferase assay.
this AlphaLISA immunoassay detects the mono-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 4.
Technical Note
AlphaLISA SIRT1 p53 lysine 382 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated p53 (368-393) peptide acetylated at lysine 382.
Technical Note
Exposing and detecting membrane and intracellular proteins: AlphaLISA simplifies the detection of HO-1
This technical document reveals the application of AlphaLISA technology in measuring HO-1 concentrations within HeLa cell extracts.
Technical Note
AlphaLISA SIRT1 Histone H3-Lysine 4 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4.
Technical Note
AlphaLISA KAT5 (TIP60) assay
This AlphaLISA™ immunodetection assay measures the acetylation of a Histone H4 (1-25) peptide at the N-terminal lysine residues.
Technical Note
Development of an AlphaLISA MEK1 kinase assay using full-length ERK2 substrate
This technical note illustrates one example of how these toolbox beads may be used to efficiently develop an assay for assessing MEK1 phosphorylation of a native ERK2 substrate.
Brochure
Alpha assays and reagents catalog
Alpha technology rapidly measures targets like enzymes, DNA, proteins, and more, in an easy-to-use homogenous format. Learn more about all the targets and reagents available.
Infographic
HTRF principle and ease of use
Discover how HTRF works, the advantages, and the wide range of targets and applications, all presented in this infographic.
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