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steadylite plus Reporter Gene Assay System, 10 mL

Steadylite is a reporter gene assay with the longest-lasting signal for quantitation of firefly luciferase in mammalian cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Reporter Gene
Assay Points 100

Steadylite is a reporter gene assay with the longest-lasting signal for quantitation of firefly luciferase in mammalian cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Overview

Steadylite plus™ is a homogeneous, high sensitivity luciferase reporter gene assay system quantitation of firefly
luciferase activity in mammalian cells. steadylite reporter assay was designed to provide an extended signal
half-life of more than 4 hours, making it detection system of choice batch processing systems where a large number
of processed plates can wait in stacks before measurement.

steadylite plus luminescence assay is suitable 96-, 384-, or 1536-well microplate formats, and is ideal for:

  • High throughput assays employing a batch processing methodology
  • Any experimental design where detection is delayed, requiring a strong, steady, and long-lived luminescent
    signal

As with all members of Revvity's Lites suite of luciferase detection reagents, steadylite plus offers superior
performance in an easy-to-use format, providing following benefits:

  • Convenient One-Step Processing: No washes required - simply mix, incubate and read
  • Enhanced Stability: All lites reagents are designed extended storage at 2-8°C
  • No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards
    and need hood work
  • Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background
    ratios and excellent Z' values

Specifications

Application
Reporter Gene
Assay Points
100
Automation Compatible
Yes
Brand
steadylite plus
Cellular or Signaling Pathway
DNA Transcription
Signal Transduction
Cellular Process
DNA Transcription
Signal Transduction
Detection Modality
Luminescence
Format
Microplates
One Unit Contains
10.0 mL
Signal Halflife
4-5 hours
Shipping Conditions
Shipped Ambient
Unit Size
100 (96-well) / 400 (384-well)

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