ProteinEXact Assay Reagent Kit

With the analytical power and speed of the LabChip GXII Touch protein characterization system and IntelliChip™ assay optimization technology, you can accelerate your protein development workflows with reproducible and sensitive protein quantitation, and an expanded sizing range.

• Dependable reproducibility for quantitation (<10% CV) and sizing (<2% CV)
• Broad sizing and quantitation range: 6.5-250 kDa and 10-2000 ng/μL
• Sensitive: 0.2 ng/μL
• Flexible: Run in batches of 48 or 96 samples at a time
• Fast: Analysis in ~65 sec per sample
• The LabChip GXII Touch protein characterization system delivers comparable results to traditional capillary electrophoresis with 46X faster run times without compromising sensitivity, resolution or reproducibility.

Choose ProteinEXact Assay for sizing and quantitation application for proteins ranging in size from 6.5 to 250 kDa.

Specifications:

• Sizing Range: 6.5 kDa - 250 kDa
• Sizing Precision RSD (CV): <2%
• Sizing Resolution: ≤10%
• Linear Dynamic Range: 10-2000 ng/µL
• Maximum Total Protein Concentration: 2 mg/mL
• Sensitivity Limit of Detection (LOD): 0.2 ng/µL
• Quantitation Reproducibility: <10%
• Maximum Salt Concentration: 1M NaCl at pH 6.5 to 8.5
• Chip Primes per Reagent Kit: 10
• Chip Sample Lifetime: 400
• Sample Analysis Time: 65 sec

ProteinEXact Assay Reproducibility & Sensitivity

Figure 1: At both high and low concentration ranges, the ProteinEXact assay provides exceptional reproducibility at a CV less than 10%

Figure 2: ProteinEXact assay is sensitive across an expanded sizing range (6.5 – 250 kDa) starting as low as 0.2 ng/µL

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the data for internal research and training purposes solely in combination with Revvity, Inc. instrumentation, and not to generate revenue, which may include, but is not limited to use of the product: (1) in manufacturing or in quality assurance or quality control of a commercial product; (2) to provide a service, information, or data for a fee or other consideration; (3) for therapeutic or prophylactic purposes; (4) for diagnostic use; and (5) for resale, whether or not such items are resold for use in research. For information on purchasing a license to this product for any other purposes, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

1. Smith, M. T., Zhang, S., Adams, T., Dipaolo, B., & Dally, J. (2017). Establishment and validation of a microfluidic capillary gel electrophoresis platform method for purity analysis of therapeutic monoclonal antibodies. Electrophoresis,38(9-10), 1353-1365. doi:10.1002/elps.201600519