PhenoVue Nile Red Lipid Stain

SDS
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS. View All
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.

PhenoVue Nile Red Lipid stain exhibits:

  • maximum excitation wavelength of 512nm and maximum emission wavelength of 585 when complexed with triglycerides
  • maximum excitation wavelength of 552nm and maximum emission wavelength of 638nm when complexed with phospholipids

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Feature Specification
Color Yellow/Orange
Filter Cy3
Organelle and Cell Compartment Lipid droplets

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.

PhenoVue Nile Red Lipid stain exhibits:

  • maximum excitation wavelength of 512nm and maximum emission wavelength of 585 when complexed with triglycerides
  • maximum excitation wavelength of 552nm and maximum emission wavelength of 638nm when complexed with phospholipids

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Product Variant
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PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red

Product information

Overview

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.

Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are ​involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.

Additional product information

Features
Numbers of vials per unit 2
Quantity or volume per vial 10mg (31.4µmoles)
Form Powder
Storage -20°C
Recommended working concentration 200nM (0.008ng/mL)​
Maximum excitation wavelength 512 nm - complexed with triglycerides
552 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
Maximum emission wavelength 585 nm - complexed with triglycerides
638 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
Common filter set Cy3.5
Live cell staining Yes
Fixed cell staining Yes. See Product Information Sheet for more information
Equivalent number of microplates 10900-32700​ x 96-well microplates
9090-32700​ x 384-well microplates
17040-40900 x 1536-well microplates

 

Specifications

Color
Yellow/Orange
Form
Powder
Maximum Emission Wavelength (Emmax)
Triglycerides: 585 nm, phospholipids: 638 nm
Maximum Excitation Wavelength (Exmax)
Triglycerides: 512 nm, phospholipids: 552 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy3
Organelle and Cell Compartment
Lipid droplets
Quantity
2 x 10 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
Room temperature or 2-8 °C, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red
PhenoVue Nile Red Lipid Stain
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Nile Red

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Product Info
PhenoVue Nile Red Lipid Stain Product Information Sheet

This is a product information sheet for PhenoVue Nile Red Lipid Stain.

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