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PhenoVue Neuronal Differentiation Staining Kit 1x384

PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit View All
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed

PhenoVue™ neuronal differentiation staining kit 1x384  comprises four validated, optimized and ready-to-use fluorescent probes to vizualize mature neurons in brain cell derived co-cultures, as well as monitor neuronal differentiation process from progenitors.

The kit is validated for use on high-content screening systems such as the Opera Phenix® Plus high-content screening system. 

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

PhenoVue™ neuronal differentiation staining kit 1x384  comprises four validated, optimized and ready-to-use fluorescent probes to vizualize mature neurons in brain cell derived co-cultures, as well as monitor neuronal differentiation process from progenitors.

The kit is validated for use on high-content screening systems such as the Opera Phenix® Plus high-content screening system. 

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product Variant
Quantity: 1 kit
Part #:
PNDIF11
For research use only. Not for use in diagnostic procedures.
Request More Information
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed

Product information

Overview

PhenoVue neuronal differentiation staining kit includes four PhenoVue probes to visualize nuclei, actin, as well as B3 tubulin and nestin proteins.

This kit can be used to assess neuron differentiation efficiency from progenitors such as embryonic stem cells or induced pluripotent stem cells (iPSC), as well as to visualize neurons in brain cell derived co-cultures.

The combination of specific, bright and photostable PhenoVue dyes enables the ability to perform a 4-plex experiment with maximum spectral separation and a significantly reduced spectral overlap.

Each PhenoVue probe has been extensively validated and carefully optimized for excellent batch-to-batch reproducibility.

The PhenoVue neuronal differentiation staining kit offers a straightforward and user-friendly protocol to facilitate the experimental procedure.

Kit Components / Quantity

  • PhenoVue Hoechst 33342 nuclear stain / 1 vial of 70 µL (500x)
  • PhenoVue Fluor 555 - Phalloidin / 1 vial (600x)
  • PhenoVue anti-B3 tubulin antibody (mouse IgG2a) / 1 vial of 100 µL (100x)
  • PhenoVue anti-nestin antibody (mouse IgG1) / 1 vial of 100 µL (100x)
  • PhenoVue Fluor 488 - Rat anti-mouse IgG1 antibody highly cross-adsorbed / 1 vial of 200 µL (50x)
  • PhenoVue Fluor 647 - Rat anti-mouse IgG2a antibody highly cross-adsorbed / 1 vial of 200 µL (50x)
  • PhenoVue dye diluent A (5X) / 1 vial - 8 mL (5X)
  • PhenoVue paraformaldehyde, 4% solution / 1 bottle of 25mL
  • PhenoVue permeabilization 0.5% Triton X-100 solution / 1 bottle of 25 mL (5X)

Specifications

Application
High Content Imaging
Microscopy
Assay Points
1 x 384-well microplate
Brand
PhenoVue™
Detection Modality
Fluorescence
Quantity
1 kit
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Kit

Image gallery

PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed
PhenoVue Neuronal Differentiation Staining Kit 1x384
Neuroblastoma SHSY5Y cells stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
Neuroblastoma SHSY5Y cells stained with PhenoVue neuronal differentiation staining kit
iPSC-derived human cortical neurons stained with anti-B3 tubulin antibody / PhenoVue Fluor 647
iPSc-derived human cortical neurons stained with PhenoVue neuronal differentiation staining kit, imaged on the Opera Phenix Plus HCS system.
iPSC-derived human cortical neurons stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary
iPSC-derived human cortical neurons stained with PhenoVue Fluor 555 - Phalloidin
Neuroblastoma SHSY5Y cells stained with anti-Nestin antibody / PhenoVue Fluor 488 secondary antibody
Neuroblastoma SHSY5Y cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 488 - Rat anti-mouse antibody IgG1 highly cross-adsorbed

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PhenoVue Neuronal Differentiation Staining Kit Product Information Sheet

This is a product information sheet for PhenoVue neuronal differentiation staining kit. View validation data, product information...

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