メニュー
Request a Quote
お問い合わせ
JP

PhenoVue Fura-2 AM, Calcium Indicator

PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator

PhenoVue™ Fura-2 AM, is a high affinity and permeable fluorescent ratiometric Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fura-2 AM Ca2+ indicator exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength shift from 336 to 365 nm upon binding to free intracellular Ca2+, and a maximum emission wavelength of 505 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Cell Function Indicator Calcium indicator
Color Green
Filter FITC

PhenoVue™ Fura-2 AM, is a high affinity and permeable fluorescent ratiometric Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fura-2 AM Ca2+ indicator exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength shift from 336 to 365 nm upon binding to free intracellular Ca2+, and a maximum emission wavelength of 505 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product Variant
For research use only. Not for use in diagnostic procedures.
Request More Information
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator
PhenoVue Fura-2 AM, Calcium Indicator

Product information

Overview

Calcium ions are important signaling molecules mediating a broad spectrum of intracellular, intercellular as well as extracellular functions. Ca2+ plays critical physiological functions such as muscle contraction, neuronal excitability, cell migration and cell growth. Intracellular Ca2+ is stored in the endoplasmic reticulum, sarcoplasmic reticulum, and the mitochondria. Upon cell surface receptors stimulation like GPCRs, calcium is released from these internal stores and its intracellular concentration increases from 100 nM to approximately 1 μM.

Intracellular Ca2+ responses have been widely investigated with fluorescent calcium probes which fluorescence signal increases upon Ca2+ binding.

The first-generation of fluorescent calcium indicators, including Fura-2, Fluo-3 and Rhod-2, were developed, followed by a second-generation, such as Fluo-4, with improved properties especially brightness and signal-to-noise ratio. However, the presence of anion transporters expressed at the cell surface may impair the intracellular retention of the fluorescent calcium indicators which are cleared out from cells. Inhibitors of these transporters, such as Probenecid, are commonly used but suffer from limitations such as cytotoxicity, off-targets, or inconsistent calcium flux kinetics. Such limitations can be overcome with third generation of fluorescent calcium indicators including Cal-520, Cal-590 and their corresponding Cal-520, Cal-590 Bright versions which do not require probenecid.

PhenoVue Fluorescent Calcium Indicators, encompassing Fura-2 AM, Fluo-4 AM, Cal-520 AM, Cal-590 AM, Cal-520 AM Bright and Cal-590 AM Bright, enable a myriad of applications as well as multiplexing capability to unravel intracellular calcium mediated functions.

PhenoVue Fura-2 AM is a high affinity cell permeable Ca2+ indicator which is rapidly metabolized by intracellular esterase, leading to a green, fluorescent signal.

Features:

  • Numbers of Vials Per Unit: 20
  • Quantity or Volume Per Vial: 50 µg (50 nmoles)
  • Form: Solid
  • Storage: -16 °C
  • Recommended Working Concentration: 5 µM
  • Maximum Excitation Wavelength: 336 nm (Ca2+ free) / 365 nm (Ca2+ bound)
  • Maximum Emission Wavelength: 505 nm
  • Live Cell Staining: Yes
  • Fixed Cell Staining: No

Filter Set:

  • Ex/Em1 = 340/510 nm cutoff 475 nm
  • Ex/Em2 = 380/510 nm cutoff 475 nm

Equivalent Number of Microplates:

  • 6-20 x 96-well microplates
  • 6-20 x 384-well microplates
  • 8-32 x 1536-well microplates

Specifications

Color
Green
Form
Solid
Maximum Emission Wavelength (Emmax)
505 nm
Maximum Excitation Wavelength (Exmax)
336 nm (Ca2+ free) / 365 nm (Ca2+ bound)
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Cell Function Indicator
Calcium indicator
Detection Modality
Fluorescence
Filter
FITC
Quantity
20 x 50 µg
Sample Type
Live samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Spectra Viewer

Resources

Are you looking for resources, click on the resource type to explore further.

1-5 of 5 Resources
Brochure Icon
Brochure
PhenoVue Cellular Imaging Reagents Brochure
Learn More
Flyer Icon
Flyer
PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

Learn More
Product Info Icon
Product Info
PhenoVue Fura-2 AM, Calcium Indicator Product Sheet

This is a product information sheet for PhenoVue Fura-2 AM, Calcium Indicator

Learn More
Product Info Icon
Product Info
PhenoVue Fura-2 AM, Calcium Indicator Product Sheet

This is a product information sheet for PhenoVue Fura-2 AM, Calcium Indicator

Learn More
Flyer Icon
Flyer
PhenoVue Reagents & Imaging Microplates Product List
Learn More
Scroll Icon