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PhenoVue Fluor 647 - Phalloidin

PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS. View All
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin

PhenoVue™ Fluor 647 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 647 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 647 - Phalloidin has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Red
Filter Cy5
Fluorophore PhenoVue™ Fluor 647
Organelle and Cell Compartment Actin

PhenoVue™ Fluor 647 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 647 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 647 - Phalloidin has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product Variant
For research use only. Not for use in diagnostic procedures.
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PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin

Product information

Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 647 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Quantity per pack: 300 units (10 nmoles; 13 µg)

Recommended working concentration: 165 nM (0.21 µg/mL)

Equivalent number of microplates:

  • 2 - 6 x 96-well microplates
  • 1.5 - 6 x 384-well microplates
  • 3 - 10 x 1536-well microplates

Specifications

Color
Red
Form
Desiccated
Maximum Emission Wavelength (Emmax)
670 nm
Maximum Excitation Wavelength (Exmax)
650 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy5
Fluorophore
PhenoVue™ Fluor 647
Organelle and Cell Compartment
Actin
Quantity
1 x 300 units
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin
PhenoVue Fluor 647 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 647 Phalloidin (actin, red). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 647 Phalloidin (actin, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 647 - Phalloidin

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PhenoVue Fluor - Phalloidin Conjugates Product Information Sheet

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