Highly cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 647.
PhenoVue Fluor 647 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 647 donkey anti-rabbit antibody, highly cross-adsorbed, has a maximum excitation wavelength of 650nm and a maximum emission wavelength of 670nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
Feature | Specification |
---|---|
Color | Red |
Filter | Cy5 |
Fluorophore | PhenoVue™ Fluor 647 |
Host Species | Donkey |
Target Species | Rabbit |
Highly cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 647.
PhenoVue Fluor 647 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 647 donkey anti-rabbit antibody, highly cross-adsorbed, has a maximum excitation wavelength of 650nm and a maximum emission wavelength of 670nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue Fluor 647 - donkey anti-rabbit IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, mouse, hamster, human, bovine, horse, or chicken serum to minimize cross-reactivity with other species.
Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 647 - donkey anti-rabbit IgG (H+L) antibody, highly cross-adsorbed.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Numbers of vials per unit | 1 |
---|---|
Quantity or volume per unit | 1 mg |
Form | Lyophilized |
Storage | 2-8°C |
Recommended working concentration | 1.5 µg/mL (10nM) |
Maximum excitation wavelength | 650 nm |
Maximum emission wavelength | 670 nm |
Common filter set | Cy5 |
Live cell staining | No |
Fixed cell staining | Yes |
Equivalent number of microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
Color |
Red
|
---|---|
Form |
Lyophilized
|
Maximum Emission Wavelength (Emmax) |
670 nm
|
Maximum Excitation Wavelength (Exmax) |
650 nm
|
Application |
High Content Imaging
Microscopy
|
---|---|
Brand |
PhenoVue™
|
Cross-Adsorption |
Highly cross-adsorbed
|
Detection Modality |
Fluorescence
|
Filter |
Cy5
|
Fluorophore |
PhenoVue™ Fluor 647
|
Host Species |
Donkey
|
Quantity |
1 x 1 mg
|
Sample Type |
Fixed samples only
|
Shipping Conditions |
Shipped Ambient
|
Storage Conditions |
2-8 °C, protected from light
|
Target Species |
Rabbit
|
Type |
Individual Reagent
|
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Donkey anti-rabbit IgG (H+L) antibodies are conjugated with our bright PhenoVue Fluor dyes.
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