PhenoVue Fluor 594 - Phalloidin

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PhenoVue Fluor 594 - Phalloidin

SDS

HeLa cells stained with PhenoVue Fluor 594 - Phalloidin

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PhenoVue Fluor 594 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 594 conjugated Phalloidin exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 594 - Phalloidin has a maximum excitation wavelength of 590 nm and a maximum emission wavelength of 617 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information

Feature	Specification
Color	Red/Orange
Filter	Texas-Red
Fluorophore	PhenoVue^™ Fluor 594
Organelle and Cell Compartment	Actin

PhenoVue Fluor 594 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 594 conjugated Phalloidin exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 594 - Phalloidin has a maximum excitation wavelength of 590 nm and a maximum emission wavelength of 617 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information

Product Variant

Quantity: 1 x 300 units

Part #:

CP25941

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Product information

Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of particular interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 594 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Additional product information

Features

Numbers of vials per unit	1
Quantity or volume per vial	300 units (10nmoles; 13µg)
Form	Dessicated
Storage	-20°C
Recommended working concentration	165nM (0.21µg/mL)
Maximum excitation wavelength	590 nm
Maximum emission wavelength	617 nm
Common filter set	FITC
Live cell staining	No
Fixed cell staining	Yes
Equivalent number of microplates	2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates

Specifications

Physical Specifications

Color	Red/Orange
Form	Desiccated
Maximum Emission Wavelength (Em_max)	617 nm
Maximum Excitation Wavelength (Ex_max)	590 nm

Other Specifications

Application	High Content Imaging Microscopy
Brand	PhenoVue™
Detection Modality	Fluorescence
Filter	Texas-Red
Fluorophore	PhenoVue^™ Fluor 594
Organelle and Cell Compartment	Actin
Quantity	1 x 300 units
Sample Type	Fixed samples only
Shipping Conditions	Shipped in Dry Ice
Storage Conditions	-16 °C or below, protected from light
Type	Individual Reagent