PhenoVue Fluor 555 - WGA

PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS. View All
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.

PhenoVue™ Fluor 555 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 555 - WGA exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 555 - WGA has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Yellow
Filter Cy3
Fluorophore PhenoVue™ Fluor 555
Organelle and Cell Compartment Golgi Apparatus

PhenoVue™ Fluor 555 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 555 - WGA exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 555 - WGA has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product Variant
Quantity: 5 x 1 mg
Part #:
CP15551
For research use only. Not for use in diagnostic procedures.
Request More Information
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.

Product information

Overview

WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

PhenoVue Fluor 555 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Additional product information

Features
Numbers of vials per unit 5
Quantity or volume per unit 1mg (29.2nmoles)​
Form Lyophilized​
Storage 2 - 8°C​
Recommended working concentration 5 µg/mL (146nM )
Maximum excitation wavelength 555nm
Maximum emission wavelength 570nm
Common filter set Cy3
Live cell staining Yes
Fixed cell staining Yes. See Product Information Sheet for more information
Equivalent number of microplates 36 - 100 x 96-well microplates
30 - 100​ x 384-well microplates
55 - 160​ x 1536-well microplates

Specifications

Color
Yellow
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
570 nm
Maximum Excitation Wavelength (Exmax)
555 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy3
Fluorophore
PhenoVue™ Fluor 555
Organelle and Cell Compartment
Golgi Apparatus
Quantity
5 x 1 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.

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PhenoVue Fluor - WGA Conjugates Product Information Sheet

Fluorescent WGA conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly...

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