PhenoVue Fluor 555 - Phalloidin

SDS
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS. View All
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin

PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Feature Specification
Color Yellow
Filter Cy3
Fluorophore PhenoVue™ Fluor 555
Organelle and Cell Compartment Actin

PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Product Variant
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PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin

Product information

Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 555 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Additional product information

Features
Numbers of vials per unit 1
Quantity or volume per unit 300 units (10nmoles; 13µg)
Form Dessicated
Storage -20°C
Recommended working concentration 165nM (0.21µg/mL)
Maximum excitation wavelength 555nm
Maximum emission wavelength 570nm
Common filter set Cy3
Live cell staining No
Fixed cell staining Yes
Equivalent number of microplates 2 - 6 x 96-well microplates
1.5 - 6 x 384-well microplates
3 - 10 x 1536-well microplates

 

Specifications

Color
Yellow
Form
Desiccated
Maximum Emission Wavelength (Emmax)
570 nm
Maximum Excitation Wavelength (Exmax)
555 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy3
Fluorophore
PhenoVue™ Fluor 555
Organelle and Cell Compartment
Actin
Quantity
1 x 300 units
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin

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PhenoVue Fluor - Phalloidin Conjugates Product Information Sheet

This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates

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