PhenoVue Fluor 488 - WGA

SDS
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA View All
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.

PhenoVue Fluor 488 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 488 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - WGA has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Feature Specification
Color Green
Filter FITC
Fluorophore PhenoVue™ Fluor 488
Organelle and Cell Compartment Golgi Apparatus

PhenoVue Fluor 488 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 488 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - WGA has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Product Variant
Quantity: 5 x 1 mg
Part #:
CP14881
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PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.

Product information

Overview

WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

PhenoVue Fluor 488 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Additional product information

Features
Numbers of vials per unit 5
Quantity or Volume Per Vial 1mg (29.2nmoles)​
Form Lyophilized​
Storage 2-8°C​
Recommended working concentration 5 µg/mL (146nM)
Maximum excitation wavelength 495 nm
Maximum emission wavelength 520 nm
Common filter set FITC​
Live cell staining Yes
Fixed cell staining Yes. See Product Information Sheet for more information
Equivalent number of microplates 36-100 x 96-well microplates
30-100​ x 384-well microplates
55-160​ x 1536-well microplates

 

Specifications

Color
Green
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
520 nm
Maximum Excitation Wavelength (Exmax)
495 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
FITC
Fluorophore
PhenoVue™ Fluor 488
Organelle and Cell Compartment
Golgi Apparatus
Quantity
5 x 1 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue Fluor 488 - WGA
HeLa fixed (PFA) cells stained with PhenoVue Fluor 488 WGA (Plasma Membrane, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.

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