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PhenoVue Fluor 488 - WGA

PhenoVue Fluor 488 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 488 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - WGA has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

Feature Specification
Color Green
Filter FITC
Fluorophore PhenoVue Fluor 488
Organelle and Cell Compartment Golgi Apparatus

PhenoVue Fluor 488 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 488 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - WGA has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

Product Variant
Quantity: 5 x 1 mg
Part #:
CP14881
For research use only. Not for use in diagnostic procedures.

Overview

WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

PhenoVue Fluor 488 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

See product information sheet for more information on fixed-cell staining.

Recommended working concentration: 5 µg/mL (146 nM)

Equivalent number of microplates:

  • 36 - 100 x 96-well microplates
  • 30 - 100 x 384-well microplates
  • 55 - 160 x 1536-well microplates

Specifications

Color
Green
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
520 nm
Maximum Excitation Wavelength (Exmax)
495 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
FITC
Fluorophore
PhenoVue Fluor 488
Organelle and Cell Compartment
Golgi Apparatus
Quantity
5 x 1 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Type
Individual Reagent

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Resources

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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Product Info
PhenoVue Fluor - WGA Conjugates Product Information Sheet

Fluorescent WGA conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly...

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PhenoVue reagents & imaging microplates product list

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