PhenoVue™ Fluor 405 – Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 405 conjugated Phalloidin exhibits bright blue fluorescence and can be multiplexed for imaging and high-content screening applications. The PhenoVue Fluor 405 conjugated Phalloidin is validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 405 conjugated Phalloidin has a maximum excitation wavelength of 410 nm and a maximum emission wavelength of 452 nm.
Feature | Specification |
---|---|
Color | Blue |
Filter | Ex: 375-430 nm, Em: 440-500 nm |
Fluorophore | PhenoVue™ Fluor 405 |
Organelle and Cell Compartment | Actin |
PhenoVue™ Fluor 405 – Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 405 conjugated Phalloidin exhibits bright blue fluorescence and can be multiplexed for imaging and high-content screening applications. The PhenoVue Fluor 405 conjugated Phalloidin is validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 405 conjugated Phalloidin has a maximum excitation wavelength of 410 nm and a maximum emission wavelength of 452 nm.
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 405 – Phalloidin can be used to visualize actin filaments in indirect immunofluorescence, immunohistochemistry as well as high-content analysis and screening applications. Its photophysical property enables extended multiplexing capability by adding a fifth color while avoiding spectral overlap and providing high brightness as well as photostability.
Quantity per pack: 300 units (10 nmoles; 11 µg)
Suggested working concentration: 165 nM (0.17 µg/mL)
Equivalent number of microplates:
Color |
Blue
|
---|---|
Form |
Desiccated
|
Maximum Emission Wavelength (Emmax) |
452 nm
|
Maximum Excitation Wavelength (Exmax) |
410 nm
|
Application |
High Content Imaging
Microscopy
|
---|---|
Brand |
PhenoVue™
|
Detection Modality |
Fluorescence
|
Filter |
Ex: 375-430 nm, Em: 440-500 nm
|
Fluorophore |
PhenoVue™ Fluor 405
|
Organelle and Cell Compartment |
Actin
|
Quantity |
1 x 300 units
|
Sample Type |
Fixed samples only
|
Shipping Conditions |
Shipped in Dry Ice
|
Storage Conditions |
-16 °C or below, protected from light
|
Type |
Individual Reagent
|
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