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PhenoVue Fluor 405 – Phalloidin

PhenoVue™ Fluor 405 – Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 405 conjugated Phalloidin exhibits bright blue fluorescence and can be multiplexed for imaging and high-content screening applications. The PhenoVue Fluor 405 conjugated Phalloidin is validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 405 conjugated Phalloidin has a maximum excitation wavelength of 410 nm and a maximum emission wavelength of 452 nm.

Feature Specification
Color Blue
Filter Ex: 375-430 nm, Em: 440-500 nm
Fluorophore PhenoVue Fluor 405
Organelle and Cell Compartment Actin

PhenoVue™ Fluor 405 – Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 405 conjugated Phalloidin exhibits bright blue fluorescence and can be multiplexed for imaging and high-content screening applications. The PhenoVue Fluor 405 conjugated Phalloidin is validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 405 conjugated Phalloidin has a maximum excitation wavelength of 410 nm and a maximum emission wavelength of 452 nm.

Product Variant
For research use only. Not for use in diagnostic procedures.
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Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 405 – Phalloidin can be used to visualize actin filaments in indirect immunofluorescence, immunohistochemistry as well as high-content analysis and screening applications. ​​Its photophysical property enables extended multiplexing capability by adding a fifth color while avoiding spectral overlap and providing high brightness as well as photostability.

Quantity per pack: 300 units (10 nmoles; 11 µg)

Suggested working concentration: 165 nM (0.17 µg/mL)

Equivalent number of microplates:

  • 2 - 6 x 96-well microplates
  • 2 - 6 x 384-well microplates
  • 3 - 10 x 1536-well microplates

Specifications

Color
Blue
Form
Desiccated
Maximum Emission Wavelength (Emmax)
452 nm
Maximum Excitation Wavelength (Exmax)
410 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Ex: 375-430 nm, Em: 440-500 nm
Fluorophore
PhenoVue Fluor 405
Organelle and Cell Compartment
Actin
Quantity
1 x 300 units
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

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