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PhenoVue DAPI nuclear stain
PhenoVue DAPI nuclear stain
PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell' nucleus.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
| Feature | Specification |
|---|---|
| Color | Blue |
| Filter | DAPI |
| Organelle and Cell Compartment | Nucleus |
PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell' nucleus.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue DAPI nuclear stain
PhenoVue DAPI nuclear stain
Product information
Overview
PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of minor groove of DNA. When excited by ultraviolet light, DAPI exhibits strong fluorescence at 455 nm. It is commonly used to stain cells’ nuclear compartment.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Additional product information
Features
| Numbers of vials per unit | 1 |
|---|---|
| Quantity or volume per unit | 1 mg (1mg/mL - 2.85mM) |
| Form | Liquid |
| Storage | -20°C |
| Recommended working concentration | 1µg/mL (10nM) |
| Maximum excitation wavelength | 358 nm |
| Maximum emission wavelength | 457 nm |
| Common filter set | DAPI |
| Live cell staining | usually not, except at high concentrations |
| Fixed cell staining | Yes |
| Equivalent number of microplates | 350-1040 x 96-well microplates 290-1040 x 384-well microplates 540-1630 x 1536-well microplates |
Specifications
| Color |
Blue
|
|---|---|
| Form |
Solution in dd H2O
|
| Maximum Emission Wavelength (Emmax) |
457 nm
|
| Maximum Excitation Wavelength (Exmax) |
348 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
DAPI
|
| Organelle and Cell Compartment |
Nucleus
|
| Quantity |
1 x 1 mg
|
| Sample Type |
Fixed samples only
|
| Shipping Conditions |
Shipped Ambient
|
| Storage Conditions |
2-8 °C for short term (6 months) or -16 °C or below for long term (> 6 months), protected from light
|
| Type |
Individual Reagent
|
Spectra Viewer
Resources
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This flyer describes Revvity's PhenoVue cellular imaging reagents.
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