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PhenoVue Cell Painting JUMP Kit 10 x 384-wells

PhenoVue™ Cell Painting JUMP Kits comprises 6 validated, pre-optimized fluorescent probes, in accordance with the JUMP consortium protocol v3, and associated diluent to streamline the cell painting workflow.

The kits offer excellent batch-to-batch reproducibility, are supplied with a step-by-step protocol, and are available in a range of pack sizes to match your throughput requirements.

PhenoVue Cell Painting JUMP kits are validated for use on high-content screening systems such as the Opera Phenix® Plus system.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Feature Specification
Quantity 1 kit

PhenoVue™ Cell Painting JUMP Kits comprises 6 validated, pre-optimized fluorescent probes, in accordance with the JUMP consortium protocol v3, and associated diluent to streamline the cell painting workflow.

The kits offer excellent batch-to-batch reproducibility, are supplied with a step-by-step protocol, and are available in a range of pack sizes to match your throughput requirements.

PhenoVue Cell Painting JUMP kits are validated for use on high-content screening systems such as the Opera Phenix® Plus system.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Overview

Cell Painting is a powerful phenotypic high-content screening approach which combines cell and computational biology to unravel cells’ responses when subjected to perturbagens.

Cell are "painted" by labelling different cellular compartments with different fluorescent bioprobes to quantitatively profile multiple phenotypic parameters in order to better understand the effects of chemical compounds, drugs, genes, or other test articles.​

PhenoVue Cell Painting JUMP Kits comprise validated, pre-optimized fluorescent bioprobes, aligned to the JUMP consortium protocol v3, to streamline your workflow, saving time and costs.

PhenoVue Cell Painting JUMP Kits provide:

  • Excellent batch-to-batch reproducibility The cell painting kit reagents are tested extensively (>80 parameters) using state-of-the-art techniques to guarantee high quality e.g. LCMS, HPLC, absorbance spectra, concentration, high-content imaging, degree of labelling (DoL), with strict acceptance criteria to deliver reliable and accurate results.
  • Ease-of-use The reagents in the cell painting kits are supplied in optimized formulations to facilitate assay set-up, together with a detailed step-by-step protocol.
  • Convenient pack sizes The cell painting kits are available in three different sizes, 1 x 384-well plate, 10 x 384-well plates and 100 x 384-well plates to meet your throughput needs and avoid waste.
  • Tried and Trusted used by the Joint Undertaking in Morphological Profiling-Cell Painting (JUMP-CP) consortium in creating the world’s largest, public cell painting dataset.
Kit Components / Quantity
  • PhenoVue Hoechst 33342 Nuclear Stain / 1 vial - 140µg
  • PhenoVue Fluor 488 - Concanavalin A / 1 vial - 1 mg
  • PhenoVue 512 Nucleic Acid Stain / 1 vial - 800nmol
  • PhenoVue Fluor 555 - WGA / 1 vial - 0.2mg
  • PhenoVue Fluor 568 - Phalloidin / 1 vial - 1nmol
  • PhenoVue 641 Mitochondrial Stain / 2 vials - 50µg per vial
  • PhenoVue Dye Diluent A (5X) / 1 vial - 80mL (5X)

Specifications

Application
High Content Imaging
Microscopy
Assay Points
10 x 384-well microplates
Brand
PhenoVue™
Detection Modality
Fluorescence
Organelle and Cell Compartment
Actin
Endoplasmic Reticulum
Golgi
Mitochondria
Nuclei
Nucleoli
Plasma membranes
Quantity
1 kit
Sample Type
Fixed samples only
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Kit

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Resources

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Whitepaper
Applying functional genomics strategies to high-content phenotypic screening

This whitepaper delves into key concepts, strategies, and best practices for leveraging high- content imaging (HCI) methods such...

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Whitepaper
Cell painting - a cellular imaging and machine learning approach to drug discovery

Phenotypic Drug Discovery Re-Imagined

Cell Painting is a phenotypic screening method and a powerful application of high-content...

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Application Note
Cell painting for phenotypic screening

Cell painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’...

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Infographic
Cell painting: from images to innovation

Cell painting is an imaging-based phenotypic profiling method that measures phenotypic features to characterize the biological...

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FAQ
Cell painting: your questions answered

With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the...

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Application Note
Phenotypic analysis of CRISPR-Cas9 cell-cycle knockouts using cell painting

Combining CRISPR gene editing and cell painting provides researchers with the ability to correlate genotypes with phenotypes and...

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