PhenoVue 578 Mitochondrial Stain

SDS
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain View All
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain

PhenoVue 578 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.

PhenoVue 578 Mitochondria stain exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 578 Mitochondrial stain has a maximum excitation wavelength of 578 nm and a maximum emission wavelength of 599 nm, which makes it an alternative to the similar stain MitoTracker™ Red CMTMRos.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Feature Specification
Color Red/Orange
Filter Texas-Red
Organelle and Cell Compartment Mitochondria

PhenoVue 578 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.

PhenoVue 578 Mitochondria stain exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 578 Mitochondrial stain has a maximum excitation wavelength of 578 nm and a maximum emission wavelength of 599 nm, which makes it an alternative to the similar stain MitoTracker™ Red CMTMRos.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

View product information
Product Variant
Request More Information
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain

Product information

Overview

PhenoVue 578 Mitochondrial stain is a cationic rosamine based structure. With mitochondrial membrane potential, it accumulates in mitochondria through electrostatic interactions and reacts with thiol moieties forming stable thioether bonds. Therefore PhenoVue 578 Mitochondrial stain is well retained after cell fixation.

Fluorescent mitochondrial stains are commonly used for visualizing and quantifying mitochondria whose dysfunctions, including impaired biogenesis, dynamics or trafficking, are a hallmark of some human diseases, such as neurodegenerative disorders.​

PhenoVue 578 Mitochondrial stain can be used to detect mitochondria in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Additional product information

Features
Numbers of vials per unit 20
Quantity or volume per vial 50µg (94nmoles)​
Form Dessicated
Storage -20°C
Recommended working concentration 100nM (0.05ng/mL)​
Maximum excitation wavelength 578 nm
Maximum emission wavelength 599 nm
Common filter set Texas-Red
Live cell staining Yes
Fixed cell staining No
Equivalent number of microplates 650-1850 x 96-well microplates
550-1950 x 384-well microplates
1000-3050 x 1536-well microplates

 

Specifications

Color
Red/Orange
Form
Desiccated
Maximum Emission Wavelength (Emmax)
599 nm
Maximum Excitation Wavelength (Exmax)
578 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Texas-Red
Organelle and Cell Compartment
Mitochondria
Quantity
20 x 50 µg
Sample Type
Live cells with fixation post staining
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Image gallery

PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain
HeLa fixed (PFA) cells stained with PhenoVue 578 Mitochondrial Stain (Mitochondria, red) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
HeLa cells stained with PhenoVue 578 Mitochondrial Stain

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