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PhenoVue 551 Mitochondrial Stain

PhenoVue 551 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.

PhenoVue 551 Mitochondrial stain exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 551 Mitochondrial stain has a maximum excitation wavelength of 551 nm and a maximum emission wavelength of 576 nm, which makes it an alternative to the similar stain MitoTracker™ Orange CMTMRos.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Feature Specification
Color Yellow
Filter Cy3
Organelle and Cell Compartment Mitochondria

PhenoVue 551 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.

PhenoVue 551 Mitochondrial stain exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 551 Mitochondrial stain has a maximum excitation wavelength of 551 nm and a maximum emission wavelength of 576 nm, which makes it an alternative to the similar stain MitoTracker™ Orange CMTMRos.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Overview

PhenoVue 551 Mitochondrial stain is a cationic rosamine based structure. With mitochondrial membrane potential, it accumulates in mitochondria through electrostatic interactions and reacts with thiol moieties forming stable thioether bonds. Therefore PhenoVue 551 Mitochondrial stain is well retained after cell fixation.

Fluorescent mitochondrial stains are commonly used for visualizing and quantifying mitochondria whose dysfunctions, including impaired biogenesis, dynamics or trafficking, are a hallmark of some human diseases, such as neurodegenerative disorders.​

PhenoVue 551 Mitochondrial stain can be used to detect mitochondria in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Additional product information

Features
Numbers of vials per unit 20
Quantity or volume per vial 50µg (117nmoles)
Form Dessicated
Storage -20°C
Recommended working concentration 100nM (0.04ng/mL)​
Maximum excitation wavelength 551 nm
Maximum emission wavelength 576 nm
Common filter set Cy3, RFP
Live cell staining Yes
Fixed cell staining No
Equivalent number of microplates 800-2400 x 96-well microplates
650-2400 x 384-well microplates
1250-3800 x 1536-well microplates

 

Specifications

Color
Yellow
Form
Desiccated
Maximum Emission Wavelength (Emmax)
576 nm
Maximum Excitation Wavelength (Exmax)
551 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy3
Organelle and Cell Compartment
Mitochondria
Quantity
20 x 50 µg
Sample Type
Live cells with fixation post staining
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

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