The NEXTFLEX  Rapid DNA-seq kit 2.0 produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.

Figure 1: Libraries prepared with NEXTFLEX  rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K’s kit using the same number of PCR cycles from a 250 ng and 1 ng input (A) and robust conversion rate (B).

Additional Data between NEXTFLEX Rapid DNA-Seq 2.0 and Competitor K’s Kit Libraries

Figure 2: Libraries prepared with NEXTFLEX  Rapid DNA-seq 2.0 shows less GC bias than libraries prepared with Competitor K’s kit.

Figure 3: Libraries prepared with NEXTFLEX  Rapid DNA-seq 2.0 shows higher genome coverage than libraries prepared with Competitor K’s kit.

Flexible Multiplexing Options

The NEXTFLEX adapters are long, annealed adapters containing indexed sequences, which offer an improved multiplexing workflow and flexible experimental setups catered to individual needs. Our barcode portfolio encompasses options for single or paired-end multiplex sequencing. The NEXTFLEX Rapid DNA-seq kit 2.0 reagents are compatible with the NEXTFLEX Unique Dual Index Barcodes for up to 1,536 sample multiplexing.

NEXTFLEX UDI-UMI adapters, incorporating molecular barcodes into each adapter, are also available for applications involving the detection of rare variants.

Automation Compatibility

The NEXTFLEX Rapid DNA-seq Kit 2.0 is compatible with multiple liquid handlers, including the Revvity Sciclone G3 NGSx and Zephyr G3 NGS workstations.