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NEXTFLEX NGS Cleanup Beads

The NEXTFLEX^® NGS Cleanup Beads are Solid-phase reversible immobilization (SPRI) beads that efficiently purify nucleic acids using reversible magnetic bead binding. They are ideal for PCR and NGS cleanup, removing unwanted components like soluble contaminants, nucleotides, primers, salts, and adapters. They selectively bind DNA by size with high recovery and consistent results. These SPRI beads can be used manually or in any automated liquid handler, depending on sample throughput.

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Feature	Specification
Automation Compatible	Yes
Product Group	Accessories

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Product variants

Unit Size: 1 mL

Part #:

NOVA-BEADS-1ML

Unit Size: 5 mL

Part #:

NOVA-BEADS-5ML

Unit Size: 50 mL

Part #:

NOVA-BEADS-50ML

Unit Size: 500 mL

Part #:

NOVA-BEADS-500ML

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

The NEXTFLEX NGS Cleanup Beads are a cost-effective solution for these applications:

NGS Library Prep protocols
Any protocols requiring the removal of unwanted components such as primers, salts or size selection of DNA
Drop-in replacement for most SPRI beads

Important attributes:

Input: Amplicons, fragmented gDNA
Applications: PCR Purification, DNA Cleanup, NGS Cleanup
Recovery: 70-90% for dsDNA >100 bp
Size Selection: Please check what Bead-to-sample Ratio is and why it matters
Storage: 4ºC

Additional product information

Features:

Drop in AMPure XP replacement: NEXTFLEX beads use the same paramagnetic SPRI chemistry
Tunable size selection : adjust the bead ratio for left , right or double side clean ups, eliminating gels or chips. Benchmarked performance matches AMPure XP and SPRIselect.
Platform compatibility: Most NGS library prep kits including NEXTFLEX®

Performance

Figure 1 shows an example of size selection performed with NEXTFLEX® NGS Cleanup and a popular competitor using different bead-to-sample ratios. Results show a predictable and consistent size selection, comparable to competition.

Figure 1. 625 ng of 50 bp DNA ladder (Promega) were purified with equal amount of AMPure® XP (Beckman) and NEXTFLEX® NGS Cleanup and run on 1% agarose gel for comparison.

Figure 2. shows final yields obtained when using same bead-to-sample ratio of either the NEXTFLEX® NGS Cleanup or a popular competitor to cleanup the libraries generated with our NEXTFLEX 16S V4 2.0 kit. Yields of both solutions are not significantly different.

Specifications

Other specifications

Automation Compatible	Yes
Product Group	Accessories
Shipping Conditions	Shipped Ambient
Unit Size	5 mL

FAQs

What are NEXTFLEX NGS Cleanup Beads used for?

NEXTFLEX NGS Cleanup Beads are SPRI-based magnetic beads used to purify DNA fragments during NGS library prep or PCR clean-up. They bind DNA (selectively by size) and remove primers, nucleotides, salts, and adapters. Use them in protocols requiring DNA cleanup or size selection.
How do NEXTFLEX NGS Cleanup Beads compare to AMPure XP beads?

NEXTFLEX beads are designed as a drop-in replacement for Beckman Coulter’s AMPure XP. In head-to-head tests, they deliver comparable DNA recovery and size-selection performance at a much lower price. Users can follow the same protocols (including bead-to-sample ratios) for PCR products or NGS library cleanup.
Can I use NEXTFLEX NGS Cleanup Beads for DNA size selection?

Yes. By adjusting the bead-to-sample ratio, NEXTFLEX beads can perform DNA size selection (e.g. enriching or depleting fragments above or below certain sizes). Revvity’s data shows predictable, consistent size cuts similar to AMPure XP or SPRIselect.
What is SPRI (Solid-Phase Reversible Immobilization) technology?

SPRI is a DNA purification method using paramagnetic beads coated with a reversible DNA-binding chemistry. When mixed with DNA and a binding buffer, the beads capture nucleic acids. A magnet pulls the beads (with DNA) aside for washing, then DNA is eluted. SPRI beads enable high recovery and scalable cleanup without centrifugation.
What DNA fragment sizes can NEXTFLEX NGS Cleanup Beads recover?

These beads efficiently recover double-stranded DNA fragments >100 bp, with typical recoveries of 70–90% for fragments above 100 bp. By adjusting the reagent ratio, you can target different size ranges (small fragments or large fragment selection) for sequencing libraries.
Are NEXTFLEX NGS Cleanup Beads compatible with automated workflows?

Yes. The beads are fully compatible with standard liquid-handling platforms (e.g. robotic workstations). They work both manually and on automation, offering the same SPRI cleanup protocol.