NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0

• Designed for low sample input – 10 ng of input cfDNA required
• Accelerated workflow with minimized hands-on time
• Flexible adapter barcode options:
  • Up to 1,536 unique dual index barcodes are available.
  • Up to 96 UDI-UMI Barcodes now available to improve the detection of rare variants.
• Compatible with Illumina® and Element Biosciences® sequencing platforms
• Protocols available for automation on the Sciclone® G3 NGSx, NGSx iQ™ and Zephyr® G3 NGS workstations

The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 delivers high coverage and reduced bias for Illumina® sequencing applications. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The availability of up to 1,536 unique dual index barcodes facilitates high-throughput applications without compromising data integrity.

Reduced Bias and Coverage Gaps

NEXTFLEX® Cell Free DNA-Seq Kit 2.0 exhibits minimal GC bias and produces uniform coverage of difficult to sequence genome regions. This kit also offers robust ligation efficiency, resulting in greater library diversity and a larger number of unique sequencing reads. These optimizations enable investigators to obtain more information from each sequencing run.

Flexible Multiplexing Options

The NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 is designed to be used with the NEXTFLEX® Unique Dual Index barcodes (384 barcodes) or the NEXTFLEX® Unique Dual Index barcodes (1,536 barcodes). NEXTFLEX UDI-UMI adapters, incorporating molecular barcodes into each adapter, are also available for applications involving the detection of rare variants.