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NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0

The NEXTFLEX® 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.

For research use only. Not for use in diagnostic procedures.

Feature Specification
Automation Compatible Yes
Product Group 16S amplicon-seq

The NEXTFLEX® 16S V4 Amplicon-Seq Library Prep Kit 2.0 is designed for the preparation of multiplexed amplicon libraries spanning the V4 hypervariable domain of microbial 16S ribosomal RNA (rRNA) genes. These libraries are compatible with paired-end sequencing on the Illumina® and Element Biosciences® sequencing platforms.

For research use only. Not for use in diagnostic procedures.

Product Variants
Unit Size: 24 rxns
Analysis
Included
Barcodes
1 - 12
Part #:
NOVA-4203-02S
Unit Size: 24 rxns
Analysis
Not Included
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1 - 12
Part #:
NOVA-4203-02
Unit Size: 96 rxns
Analysis
Included
Barcodes
1 - 48
Part #:
NOVA-4203-03S
Unit Size: 96 rxns
Analysis
Not Included
Barcodes
1 - 48
Part #:
NOVA-4203-03
Unit Size: 192 rxns
Analysis
Included
Barcodes
1 - 96
Part #:
NOVA-4203-04S
Unit Size: 192 rxns
Analysis
Not Included
Barcodes
1 - 96
Part #:
NOVA-4203-04
Unit Size: 192 rxns
Analysis
Included
Barcodes
97 - 192
Part #:
NOVA-4203-05S
Unit Size: 192 rxns
Analysis
Not Included
Barcodes
97 - 192
Part #:
NOVA-4203-05
Unit Size: 192 rxns
Analysis
Included
Barcodes
193 - 288
Part #:
NOVA-4203-06S
Unit Size: 192 rxns
Analysis
Not Included
Barcodes
193 - 288
Part #:
NOVA-4203-06
Unit Size: 192 rxns
Analysis
Included
Barcodes
289 - 384
Part #:
NOVA-4203-07S
Unit Size: 192 rxns
Analysis
Not Included
Barcodes
289 - 384
Part #:
NOVA-4203-07
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Overview

16S V4 rRNA library prep for broad-range analysis of mixed microbial populations
  • Fast and easy library prep protocol
  • Low input – Only 1 ng of input required
  • Multiplex up to 384 libraries
  • Custom sequencing primers are not required
  • Automation protocols are now available for the Sciclone® NGS and NGSx Workstations to automate your 16S sequencing
  • Functionally tested with Illumina® sequencing platforms

Analysis Powered by CosmosID HUB®

The NEXTFLEX 16S V4 rRNA Amplicon-seq panels are now available bundled with access to CosmosID-HUB; an online software solution that enables fast and easy analysis of complex microbiome data. CosmosID-HUB gives scientists user-friendly access to version-controlled and validated 16S pipelines. The machine-learning powered software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about CosmosID-HUB.

Revvity 16S rRNA Analysis - Online Demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the CosmosID-Hub® which is now available with Revvity’s NEXTFLEX™ 16S rRNA Amplicon-seq Kits. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

Specifications

Analysis
Included
Automation Compatible
Yes
Barcodes
289 - 384
Product Group
16S amplicon-seq
Shipping Conditions
Shipped in Dry Ice
Unit Size
192 rxns

Resources

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1-4 of 4 Resources
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Application Note
Automated homogenization and DNA extraction from murine GI tract tissue, cultures, and mock community for 16S sequencing

The success of sensitive downstream genomic applications such as qPCR depend on robust and reliable upstream sample preparation...

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End-to-end metagenomics solutions.

This flyer showcases Revvity's metagenomic portfolio as well as CosmosID's award winning analysis solution.

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Infographic
Limitations in metagenomics analysis: a roadblock to discoveries

Metagenomics holds tremendous promise, yet it is not without its constraints, especially when it comes to the scarcity of powerful...

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Flyer
Simplify your 16S library prep and analysis.

This flyer provides guidance on which NEXTFLEX metagenomic kit to use for microbial community profiling projects

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